# MACS2 narrowpeaks results are empty when adding an input

**URL:** https://help.galaxyproject.org/t/macs2-narrowpeaks-results-are-empty-when-adding-an-input/8195
**Category:** usegalaxy.org support
**Tags:** macs2, epigenetics
**Created:** [June 14, 2022, 2:32pm UTC](https://help.galaxyproject.org/t/macs2-narrowpeaks-results-are-empty-when-adding-an-input/8195 "2022-06-14T14:32:05Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![nourmahfel](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/nourmahfel/32/3416_2.png) [@nourmahfel](https://help.galaxyproject.org/u/nourmahfel)
#### Post date: [June 14, 2022, 2:32pm UTC](https://help.galaxyproject.org/t/macs2-narrowpeaks-results-are-empty-when-adding-an-input/8195/1 "2022-06-14T14:32:05Z")

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I have been struggling with running MACS2 for the past two weeks. The software is not producing narrowpeaks results and bed results when adding an input. I am not sure what is the issue and I have tried running it many times with different parameters. I have checked EBI/SRA and the data is loaded correctly, which consists of Input, TEAD1, and TEAD4 immunoprecipitated samples. Could you please provide an insight to the issue? I am obtaining results without an input file, but I know it is not recommended.

The link to my history is below:

[https://usegalaxy.org/u/nourmahfel/h/chip-seq](https://usegalaxy.org/u/nourmahfel/h/chip-seq)

BW,

Nour

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### Author: ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)
#### Post date: [June 16, 2022, 1:49am UTC](https://help.galaxyproject.org/t/macs2-narrowpeaks-results-are-empty-when-adding-an-input/8195/2 "2022-06-16T01:49:31Z")

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Reply at: [MACS2 scientific result troubleshooting](https://help.galaxyproject.org/t/macs2-scientific-result-troubleshooting/8200)

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### Author: ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)
#### Post date: [June 16, 2022, 1:49am UTC](https://help.galaxyproject.org/t/macs2-narrowpeaks-results-are-empty-when-adding-an-input/8195/3 "2022-06-16T01:49:37Z")

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