# MACS2 peak calling, there is not a explicit input control
**URL:** https://help.galaxyproject.org/t/macs2-peak-calling-there-is-not-a-explicit-input-control/9096
**Category:** Uncategorized
**Tags:** gtn-tutorial, macs2
**Created:** [November 27, 2022, 12:54am UTC](https://help.galaxyproject.org/t/macs2-peak-calling-there-is-not-a-explicit-input-control/9096 "2022-11-27T00:54:18Z")
**Posts on this page:** 3
**Page:** 1
### Author:  [@Pablin](https://help.galaxyproject.org/u/Pablin)
#### Post date: [November 27, 2022, 12:54am UTC](https://help.galaxyproject.org/t/macs2-peak-calling-there-is-not-a-explicit-input-control/9096/1 "2022-11-27T00:54:18Z")
Dear community,
I read that control is important to get reliable peaks when using MACS2. However, I do not find any control for my treatment regarding the experiment that I am analyzing. I am using one dataset found in Chip Atlas and they get peak calling reports for the sample. But I do not know how do they define this control. The paper mentioned they used MACS2 but no explicit input controls were given. How is usually the best way to proceed when you lack this information without biasing the results or doing too much data manipulation? Thank you in advance for any helpful information.
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### Author:  [@igor](https://help.galaxyproject.org/u/igor)
#### Post date: [November 28, 2022, 8:06am UTC](https://help.galaxyproject.org/t/macs2-peak-calling-there-is-not-a-explicit-input-control/9096/2 "2022-11-28T08:06:53Z")
Hi @Pablin
GTN provides at least couple tutorials for MACS2
> **[Galaxy Training: ATAC-Seq data analysis](https://training.galaxyproject.org/training-material/topics/epigenetics/tutorials/atac-seq/tutorial.html)**
>
> DNA methylation is an epigenetic mechanism used by higher...
> **[Galaxy Training: Identification of the binding sites of the T-cell acute l...](https://training.galaxyproject.org/training-material/topics/epigenetics/tutorials/tal1-binding-site-identification/tutorial.html)**
>
> DNA methylation is an epigenetic mechanism used by higher...
Hope it helps.
Kind regards,
Igor
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### Author:  [@Pablin](https://help.galaxyproject.org/u/Pablin)
#### Post date: [November 28, 2022, 2:35pm UTC](https://help.galaxyproject.org/t/macs2-peak-calling-there-is-not-a-explicit-input-control/9096/3 "2022-11-28T14:35:11Z")
Thank you Igor. I will have a look at it.
Pablo