# Metatranscriptomic analysis fails to determine pathwasy with HUMAnN

**URL:** <https://help.galaxyproject.org/t/metatranscriptomic-analysis-fails-to-determine-pathwasy-with-humann/18426>\
**Category:** usegalaxy.eu support\
**Tags:** troubleshooting, transcriptomics\
**Created:** [September 28, 2026, 11:42am UTC](https://help.galaxyproject.org/t/metatranscriptomic-analysis-fails-to-determine-pathwasy-with-humann/18426 "2026-09-28T11:42:14Z")\
**Posts on this page:** 1\
**Showing post:** 4

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [October 3, 2026, 12:29am UTC](https://help.galaxyproject.org/t/metatranscriptomic-analysis-fails-to-determine-pathwasy-with-humann/18426/4 "2026-10-03T00:29:48Z")

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Hi @jenny.gamezroessler

Thanks for explaining more. As you probably know, KEGG is not independently available for free for non-academic use, so this impacts what we are able to publish on the public Galaxy servers. This topic has a few more details. → [Request to enable KEGG and Pfam databases: licensing and KEGG - #2 by jennaj](https://help.galaxyproject.org/t/request-to-enable-kegg-and-pfam-databases-licensing-and-kegg/16473/2) with the direct link here. → [KEGG - Copyright and Disclaimer](https://www.kegg.jp/kegg/legal.html).

For your data, there is either actually no pathway information available for your hits, or there could be some technical issue that could be adjusted to better capture a result. We have another example in this tutorial step if you want to compare approaches. (Note that the downsampled training example didn’t have a pathway identified either!).

- [Hands-on: Analyses of metagenomics data - The global picture / Analyses of metagenomics data - The global picture / Microbiome](https://galaxyproject.github.io/training-material/topics/microbiome/tutorials/general-tutorial/tutorial.html#extraction-of-functional-information) (# extraction-of-functional-information).

Would you like help reviewing? You are welcome to [share your history](https://training.galaxyproject.org/training-material/faqs/galaxy/histories_sharing.html) back as a reply! 🙂

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_[View the full topic](https://help.galaxyproject.org/t/metatranscriptomic-analysis-fails-to-determine-pathwasy-with-humann/18426)._