# Minimap2 Memory Issue with hg38/T2T on Galaxy

**URL:** <https://help.galaxyproject.org/t/minimap2-memory-issue-with-hg38-t2t-on-galaxy/14739>\
**Category:** usegalaxy.org support\
**Tags:** troubleshooting, exceeds-memory-error\
**Created:** [February 14, 2025, 9:28pm UTC](https://help.galaxyproject.org/t/minimap2-memory-issue-with-hg38-t2t-on-galaxy/14739 "2025-02-14T21:28:26Z")\
**Posts on this page:** 1\
**Showing post:** 2

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [February 14, 2025, 9:43pm UTC](https://help.galaxyproject.org/t/minimap2-memory-issue-with-hg38-t2t-on-galaxy/14739/2 "2025-02-14T21:43:42Z")

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Welcome @jagoda_33

This is how to interpret this error. It is unrelated to how much storage space is in your account.

> [@Proteogenomics fasta file generation w CustomProDB](https://help.galaxyproject.org/t/proteogenomics-fasta-file-generation-w-customprodb/14700/2):
>
> This error message usually means that there is a data or parameter problem to resolve. It can happen with any tool. In short, the requested analysis completely overwhelmed the underlying tool and it quit out without more details.
> 
> - [FAQ: Understanding ‘exceeds memory allocation’ error messages](https://training.galaxyproject.org/training-material/faqs/galaxy/troubleshooting_excess_memory.html)
> - #exceeds-memory-error

* * *

For your case, there could be a few reasons:

1. Not enough QA done on the reads, and the tool was able to handle the data anyway with smaller genomes, but larger genomes lead to problems.

2. The current parameters are not working well for the human genomes, or the human T2T assembly in particular. More about why that could be is in here. → [Reference genomes at public Galaxy servers: GRCh38/hg38 example](https://help.galaxyproject.org/t/reference-genomes-at-public-galaxy-servers-grch38-hg38-example/11616)

Are you trying to screen out the host genome? You could try using a tool like BLASTN instead to see what happens.

Then, if you are still getting an error, is there a GTN tutorial that walks through those steps you could use as an example? If you share the types of reads and what you are trying to do, we can help to point you to the right place.

- Review the analysis domains here. → [https://training.galaxyproject.org/](https://training.galaxyproject.org/)

Tutorials are linked from tool form, too, if you want to search that way. Scroll all the way down into the Help section to find these.

- Tutorials for [**Map with minimap2** : A fast pairwise aligner for genomic and spliced nucleotide sequences](https://training.galaxyproject.org/training-material/by-tool/iuc/minimap2/minimap2.html)

We also have QA tutorials. Some are included in with the primary tutorials since sometimes the analysis domain has specific considerations, but this one has a good overview to start with.

- [Hands-on: Quality Control / Quality Control / Sequence analysis](https://training.galaxyproject.org/training-material/topics/sequence-analysis/tutorials/quality-control/tutorial.html)

Let’s start there and we can follow up! A shared history is best when reviewing an error. How to share is in the banner topic of this forum, also here.

- [How to get faster help with your question](https://help.galaxyproject.org/t/how-to-get-faster-help-with-your-question/11469)

Thanks! 🙂

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