# MITOS2 error can't run job

**URL:** <https://help.galaxyproject.org/t/mitos2-error-cant-run-job/16174>\
**Category:** Resources\
**Tags:** tool-help, mitos2\
**Created:** [August 15, 2025, 10:00am UTC](https://help.galaxyproject.org/t/mitos2-error-cant-run-job/16174 "2025-08-15T10:00:16Z")\
**Posts on this page:** 1\
**Showing post:** 2

<div class="post-metadata">

**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [August 15, 2025, 7:54pm UTC](https://help.galaxyproject.org/t/mitos2-error-cant-run-job/16174/2 "2025-08-15T19:54:04Z")

</div>

Welcome @bioinfokg

Thanks for sharing the parameters! It looks like you are using the default reference index with the most current tool version. And, the topic was tagged as being for [UseGalaxy.org](http://UseGalaxy.org), so that is where I am cross testing, but you can clarify if you are working somewhere else?

This is my test using the same configurations, and it ran successfully, so I think the tool and the reference index are working Ok.

- [https://usegalaxy.org/u/jen-galaxyproject/h/test-mitos2-https-help-galaxyproject-org-t-mitos2-error-cant-run-job-16174](https://usegalaxy.org/u/jen-galaxyproject/h/test-mitos2-https-help-galaxyproject-org-t-mitos2-error-cant-run-job-16174)

My best guess is that there is some problem with your input fasta dataset. Maybe the format? You could run the data through a tool like **NormalizeFasta** to see if that helps. Other problems could be based on the scientific content – maybe a different reference index would be a better choice?

If that is not enough help to solve this, you are welcome to [share more details](https://help.galaxyproject.org/t/how-to-get-faster-help-with-your-question/11469) and we can troubleshoot more. 🙂

---

_[View the full topic](https://help.galaxyproject.org/t/mitos2-error-cant-run-job/16174)._