# MITOS2 - Metadata erro

**URL:** <https://help.galaxyproject.org/t/mitos2-metadata-erro/13399>\
**Category:** Uncategorized\
**Tags:** tool-help, mitos2\
**Created:** [September 8, 2024, 5:31pm UTC](https://help.galaxyproject.org/t/mitos2-metadata-erro/13399 "2024-09-08T17:31:51Z")\
**Posts on this page:** 1\
**Showing post:** 2

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [September 9, 2024, 8:05pm UTC](https://help.galaxyproject.org/t/mitos2-metadata-erro/13399/2 "2024-09-09T20:05:28Z")

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Welcome, @abarrientos

I have an example of the tool use in this history →

> [@zero scores for rrnS and rrnL genes in MITOS2](https://help.galaxyproject.org/t/zero-scores-for-rrns-and-rrnl-genes-in-mitos2/12944/6):
>
> See → [https://usegalaxy.eu/u/jenj/h/test-mitos](https://usegalaxy.eu/u/jenj/h/test-mitos)

Maybe comparing to my example helps?

In short, the tool expects a single fasta sequence as the input. Your error suggests that more than one fasta sequence was input but that’s just a guess!

_Quote from the tool (scroll down to find this)_

> **Help**
> 
> MITOS2 does a de-novo annotation of a given metazoan mitochondrial sequence.
> 
> **Inputs**
> 
> - A fasta formatted sequence
> - MITOS2 processes only FASTA files containing a single sequence. If you have a FASTA file with multiple sequences you may use the tool _Split Fasta files into a collection_. The resulting collection can then used as input to MITOS2.
> 
> - The correct genetic code needs to be selected

Please give that a review, and if you need more help, please share back your history and we can try to solve whatever may be going wrong. How to share you work (with context) is in the banner at this forum, or please see here directly → [How to get faster help with your question](https://help.galaxyproject.org/docs?topic=11469)

Let’s start there, thanks! 🙂

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