# NCBI Eukaryotic Genome Annotation Pipeline (EGAP) **URL:** \ **Category:** Uncategorized\ **Tags:** development-tools, genome-annotation\ **Created:** [March 11, 2026, 12:46pm UTC](https://help.galaxyproject.org/t/ncbi-eukaryotic-genome-annotation-pipeline-egap/17561 "2026-03-11T12:46:36Z")\ **Posts on this page:** 5\ **Page:** 1
**Author:** ![Attiq\_Rehman](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/attiq_rehman/32/8489_2.png) [@Attiq\_Rehman](https://help.galaxyproject.org/u/Attiq_Rehman)\ **Post date:** [March 10, 2026, 1:26pm UTC](https://help.galaxyproject.org/t/ncbi-eukaryotic-genome-annotation-pipeline-egap/17561/1 "2026-03-10T13:26:39Z")
I would be highly obliged if some could help me annotate the Genome. Please reply and we will connect. Thanks ---
**Author:** ![Attiq\_Rehman](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/attiq_rehman/32/8489_2.png) [@Attiq\_Rehman](https://help.galaxyproject.org/u/Attiq_Rehman)\ **Post date:** [March 11, 2026, 12:46pm UTC](https://help.galaxyproject.org/t/ncbi-eukaryotic-genome-annotation-pipeline-egap/17561/2 "2026-03-11T12:46:36Z")
Anyone willing to offer some help ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [March 11, 2026, 6:22pm UTC](https://help.galaxyproject.org/t/ncbi-eukaryotic-genome-annotation-pipeline-egap/17561/3 "2026-03-11T18:22:27Z")
Hello @Attiq_Rehman Are you asking about this tool at [UseGalaxy.org](http://UseGalaxy.org)? - **NCBI EGAPx** annotates eukaryotic genomes(Galaxy Version 0.5.0+galaxy0) ([link at ORG](https://usegalaxy.org/root?tool_id=toolshed.g2.bx.psu.edu/repos/richard-burhans/ncbi_egapx/ncbi_egapx/0.5.0+galaxy0)) If yes, the tool is still experimental and not quite ready for production (general) use. The Help on the tool form explains the usage and a demonstration example is included. You could explore that to see how the development project functions. Have you tried this yet? If your larger goal is to annotate an assembly, then following the protocols here instead would be a another choice that you could use right now in Galaxy. - [Genome Annotation / Tutorial List](https://galaxyproject.github.io/training-material/topics/genome-annotation/) - [Learning Pathway: Genome annotation for eukaryotes](https://galaxyproject.github.io/training-material/learning-pathways/genome-annotation-eukaryote.html) - [Learning Pathway: Genome annotation for prokaryotes](https://galaxyproject.github.io/training-material/learning-pathways/genome-annotation-prokaryote.html) Would you like to share more about your goals? Thanks! πŸ™‚ ---
**Author:** ![Attiq\_Rehman](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/attiq_rehman/32/8489_2.png) [@Attiq\_Rehman](https://help.galaxyproject.org/u/Attiq_Rehman)\ **Post date:** [March 11, 2026, 6:50pm UTC](https://help.galaxyproject.org/t/ncbi-eukaryotic-genome-annotation-pipeline-egap/17561/4 "2026-03-11T18:50:28Z")
Thank you Jennaj, I have not tried yet. look bit complicated for me non bioinformatician. Our goal is to annotate genome available in GenBank. I requested Refseq but they said Protzoan annotation is not their preference at the moment. Would any chance you can help? ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [March 11, 2026, 8:07pm UTC](https://help.galaxyproject.org/t/ncbi-eukaryotic-genome-annotation-pipeline-egap/17561/5 "2026-03-11T20:07:14Z")
Hi @Attiq_Rehman Thank you for for explaining more! We don’t offer bioinformatic services, only the platform and tools for scientist to run their own analysis. Annotating a genome is a common task and we definitely have the training, tools, _and_ computational resources for this type of project, all made accessible for biologists. If you have questions as you work through any tutorial, or technical issues with your own data later on, you can ask here by sharing what you have so far and we’ll try to help! I would suggest starting with an orientation. - [Learning Pathway: Genome annotation for eukaryotes](https://galaxyproject.github.io/training-material/learning-pathways/genome-annotation-eukaryote.html) Then, proceed to working with your own species. This Maker protocol bundles most of what EPAG is doing. - [Hands-on: Genome annotation with Maker / Genome annotation with Maker / Genome Annotation](https://galaxyproject.github.io/training-material/topics/genome-annotation/tutorials/annotation-with-maker/tutorial.html) We have a training event in May if you would like to join us then, too! πŸŽ“ [Galaxy Training Academy 2026](https://training.galaxyproject.org/training-material/events/2026-05-18-galaxy-academy.html)πŸ“… May 18 – 22, 2026