# No results at LEfSe analysis **URL:** \ **Category:** usegalaxy.org support\ **Tags:** lefse, galaxy-biobakery\ **Created:** [January 5, 2023, 8:17am UTC](https://help.galaxyproject.org/t/no-results-at-lefse-analysis/9245 "2023-01-05T08:17:19Z")\ **Posts on this page:** 3\ **Page:** 1
**Author:** ![m.mihula](https://avatars.discourse-cdn.com/v4/letter/m/cdc98d/32.png) [@m.mihula](https://help.galaxyproject.org/u/m.mihula)\ **Post date:** [January 5, 2023, 8:17am UTC](https://help.galaxyproject.org/t/no-results-at-lefse-analysis/9245/1 "2023-01-05T08:17:19Z")
Dear all, I am using a LEfSe analysis for my sequencing data. I use this format pasted to .tsv file in the notepad: ![image](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/1/1c2a6b320f0f47c20ed0661fe9662c31f94e834e.png) Nevertheless, I do not receive any results. I got that there does not have to be any significant difference among taxons, but I have tried a lot of variable values to obtain any result and to confirm that I work correctly and the input file is ok. So I have tried every combination of “Set the strategy for multi-class analysis” and “Do you want the pairwise comparisons among subclasses to be…” at the step B (LDA Effect Size) and after that didn’t do anything, I setup both Alpha values to 1. In my opinion, that means I should see at least some differences. But I do not. So I would like to ask you if I am performing this analysis correctly and if so, what can be the troubleshoot? Thank you a lot! Martin ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [January 14, 2023, 12:39am UTC](https://help.galaxyproject.org/t/no-results-at-lefse-analysis/9245/2 "2023-01-14T00:39:39Z")
Hi @m.mihula If you are working with this tool at a public Galaxy site, is it this one? If so, you’ll need to contact them directly for usage issues. Click into this post – it explains why and how to reach them. > [@Problems with Plot Cladogram -- http://huttenhower.sph.harvard.edu/galaxy/](https://help.galaxyproject.org/t/problems-with-plot-cladogram-http-huttenhower-sph-harvard-edu-galaxy/5877/2): > > Hi @liuyushu The tool you are using is specific to the http://huttenhower.sph.harvard.edu/galaxy/ domain-specific public Galaxy server. The tools and protocols hosted there differ significantly from the Lefse tools hosted at most other public Galaxy servers (including usegalaxy.\* servers). The administrators of that particular public Galaxy server do not track this forum. Instead, contact them directly, after double-checking your protocol and inputs against the help/tutorials they offer (belo… ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [January 24, 2023, 6:46pm UTC](https://help.galaxyproject.org/t/no-results-at-lefse-analysis/9245/3 "2023-01-24T18:46:48Z")
Closed on January 24, 2023, 6:46pm UTC