# PARalyzer PAR-CLIP data analysis error: \* is not found in the .2bit file

**URL:** <https://help.galaxyproject.org/t/paralyzer-par-clip-data-analysis-error-is-not-found-in-the-2bit-file/1651>\
**Category:** Uncategorized\
**Created:** [July 1, 2019, 1:18pm UTC](https://help.galaxyproject.org/t/paralyzer-par-clip-data-analysis-error-is-not-found-in-the-2bit-file/1651 "2019-07-01T13:18:32Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Xiao](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/xiao/32/723_2.png) [@Xiao](https://help.galaxyproject.org/u/Xiao)\
**Post date:** [July 1, 2019, 1:18pm UTC](https://help.galaxyproject.org/t/paralyzer-par-clip-data-analysis-error-is-not-found-in-the-2bit-file/1651/1 "2019-07-01T13:18:32Z")

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Dear Users,

I tried to process my PAR-CLIP dataset with PARalyzer version 1.5 ([https://ohlerlab.mdc-berlin.de/software/PARalyzer\_85/](https://ohlerlab.mdc-berlin.de/software/PARalyzer_85/)). While I could start running the program without any problem, at the final stage I always got an error as below:

Running PARalyzer v1.5

Parsing SAM file(s)…Done

Creating Read Groups & Clusters…Exception in thread “main” java.lang.Exception: \* is not found in the .2bit file

at MyTwoBitParser.loadChromosome(MyTwoBitParser.java:58)

at MyTwoBitParser.getSequence(MyTwoBitParser.java:118)

at PARalyze.main(PARalyze.java:204)

I generated by 2bit file by faToTwoBit tools (e.g. faToTwoBit hg19.fasta hg19.2bit).

I really appreciate if you could have any input in this issue.

Thanks,

Xiao