# PICRUSt2 Metagenome prediction

**URL:** <https://help.galaxyproject.org/t/picrust2-metagenome-prediction/17431>\
**Category:** usegalaxy.eu support\
**Tags:** picrust2\_metagenome\_pipeline\
**Created:** [February 7, 2026, 3:57am UTC](https://help.galaxyproject.org/t/picrust2-metagenome-prediction/17431 "2026-02-07T03:57:31Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![Peter\_Janampa](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/peter_janampa/32/7271_2.png) [@Peter\_Janampa](https://help.galaxyproject.org/u/Peter_Janampa)\
**Post date:** [February 7, 2026, 3:57am UTC](https://help.galaxyproject.org/t/picrust2-metagenome-prediction/17431/1 "2026-02-07T03:57:31Z")

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Hello, is there an example of an input based on the **shared format**? Specifically, should the **shared file** also contain **FASTA sequences**?

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [February 7, 2026, 11:11pm UTC](https://help.galaxyproject.org/t/picrust2-metagenome-prediction/17431/2 "2026-02-07T23:11:47Z")

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Welcome @Peter_Janampa

It seems this prior issue is pending a correction. → [Problem on Picrust2 webtool, even with tutorial files](https://help.galaxyproject.org/t/problem-on-picrust2-webtool-even-with-tutorial-files/11412)

I’ve updated the ticket to include a link to this topic and the updated testing history originally used. You are welcome to review the history for a format example! For the technical specification, you can start here [http://biom-format.org/](http://biom-format.org/).

Any tool in Galaxy that creates the same format will be following the official specification above. To find tools to create, or manipulate biom files, including the `shared.biom` format, search the tool panel with the keyword “biom”.

Then, “shared” files in the context of the `Mothur` tool suite have a specific format. Many examples are in the tutorial here, and I’ve linked place where the plain format is include along with some explaintations.

- [Hands-on: 16S Microbial Analysis with mothur (extended) / 16S Microbial Analysis with mothur (extended) / Microbiome](https://training.galaxyproject.org/training-material/topics/microbiome/tutorials/mothur-miseq-sop/tutorial.html)
- Output of that tutorial’s workflow run on the tutorial data → [https://usegalaxy.org/workflows/invocations/1641bb092161e9ab](https://usegalaxy.org/workflows/invocations/1641bb092161e9ab)
- click into dataset 124 for a `mothur.shared` file. It is a special tabular format with headers and columns of datapoints. A tools like **Make.biom** and **Biom.info** are packing and unpacking information from the original underlying `biom` data.

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Let’s see if the EU administrators can offer some feedback at their ticket! Even correct formats are failing right now. Hopefully that can be adjusted. 🙂

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [February 12, 2026, 6:20pm UTC](https://help.galaxyproject.org/t/picrust2-metagenome-prediction/17431/3 "2026-02-12T18:20:39Z")

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Let’s close this out and consolidate into the topic with the tracking ticket. → [Problem on Picrust2 webtool, even with tutorial files - #10 by jennaj](https://help.galaxyproject.org/t/problem-on-picrust2-webtool-even-with-tutorial-files/11412/10)

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [February 12, 2026, 6:20pm UTC](https://help.galaxyproject.org/t/picrust2-metagenome-prediction/17431/4 "2026-02-12T18:20:45Z")

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [February 12, 2026, 8:06pm UTC](https://help.galaxyproject.org/t/picrust2-metagenome-prediction/17431/5 "2026-02-12T20:06:49Z")

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Update: we believe the tool is corrected as of late yesterday! Please also give your rerun a try and let us know how that goes!