# Problem to make a bacterial genome assembly database for blast

**URL:** <https://help.galaxyproject.org/t/problem-to-make-a-bacterial-genome-assembly-database-for-blast/1999>\
**Category:** Uncategorized\
**Tags:** troubleshooting, exceeds-memory-error\
**Created:** [August 28, 2019, 1:13pm UTC](https://help.galaxyproject.org/t/problem-to-make-a-bacterial-genome-assembly-database-for-blast/1999 "2019-08-28T13:13:09Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Guangzhi\_Zhang](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/guangzhi_zhang/32/873_2.png) [@Guangzhi\_Zhang](https://help.galaxyproject.org/u/Guangzhi_Zhang)\
**Post date:** [August 28, 2019, 1:13pm UTC](https://help.galaxyproject.org/t/problem-to-make-a-bacterial-genome-assembly-database-for-blast/1999/1 "2019-08-28T13:13:09Z")

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Hi,  
I am trying to make a local database with bacterial genomes assembled ourself ( about 200 genomes) with the NCBI Blast+makeblasedb tool, However, I am keeping get error message, any suggestion?

Thanks,

Guangzhi

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [August 28, 2019, 10:07pm UTC](https://help.galaxyproject.org/t/problem-to-make-a-bacterial-genome-assembly-database-for-blast/1999/2 "2019-08-28T22:07:59Z")

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Welcome @Guangzhi_Zhang

Guide for troubleshooting problems: [Troubleshooting resources for errors or unexpected results](https://help.galaxyproject.org/t/troubleshooting-resources-for-errors-or-unexpected-results/42)

If your error is not covered there, the job may be still be actually too large to execute or there is an input problem. Error messages can vary.

This recent Q&A (and many others) explain how to review errors: [DexSeq Count issue -- Job failing for exceeding memory resources: input problem or actually too large to execute at public server](https://help.galaxyproject.org/t/dexseq-count-issue-job-failing-for-exceeding-memory-resources-input-problem-or-actually-too-large-to-execute-at-public-server/1997/2)

I would suggest starting by checking that your input fasta is formatted correctly. Run the tool `NormalizeFasta` with the options wrap at 80 bases and trim the title line at the first white-space. The rules for formatting a Custom genome will apply for this case as well and has an FAQ:

- Support hub: [https://galaxyproject.org/support/](https://galaxyproject.org/support/)
- [Preparing and using a Custom Reference Genome or Build](https://galaxyproject.org/learn/custom-genomes/)

My guess is that the data is actually too large given what you state. But it is worth checking the format first before exploring other Galaxy choices.

- [My job ended with an error. What can I do?](https://galaxyproject.org/support/tool-error/) – even if your error message does not match up exactly, the help can still aid in resolving problems.
- Galaxy choices summarized (if the job is truly too large to run at whatever public server you are using): [https://galaxyproject.org/choices/](https://galaxyproject.org/choices/)
- Ways to use Galaxy, including setting up your own server with sufficient resources to work with such large data: [https://galaxyproject.org/use/](https://galaxyproject.org/use/)

Thanks!