# problem with "Download and Extract Reads in FASTA/Q format from NCBI SRA” for local galaxy

**URL:** <https://help.galaxyproject.org/t/problem-with-download-and-extract-reads-in-fasta-q-format-from-ncbi-sra-for-local-galaxy/2056>\
**Category:** Uncategorized\
**Tags:** download, sra, galaxy-local\
**Created:** [September 7, 2019, 11:57pm UTC](https://help.galaxyproject.org/t/problem-with-download-and-extract-reads-in-fasta-q-format-from-ncbi-sra-for-local-galaxy/2056 "2019-09-07T23:57:17Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![spz1st](https://avatars.discourse-cdn.com/v4/letter/s/8dc957/32.png) [@spz1st](https://help.galaxyproject.org/u/spz1st)\
**Post date:** [September 7, 2019, 11:57pm UTC](https://help.galaxyproject.org/t/problem-with-download-and-extract-reads-in-fasta-q-format-from-ncbi-sra-for-local-galaxy/2056/1 "2019-09-07T23:57:17Z")

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I just installed galaxy (release\_19.05) on a local server (EL7) under the account galaxy and was able to install some tools. But when I tried to download some sequences from NCBI SRA, I got problems. The process got hung. When I checked processes of galaxy, I saw the following two processes.

```
galaxy 90075 90070 0 19:26 ? 00:00:00 prefetch -X 200G --ascp-path /usr/local/bin/ascp|/usr/local/bin/asperaweb_id_dsa.openssh SRR3541303
galaxy 90076 90075 0 19:26 ? 00:00:00 /usr/local/bin/ascp -i /usr/local/bin/asperaweb_id_dsa.openssh -pQTk1 -l 450m dbtest@sra-download.ncbi.nlm.nih.gov:data/sracloud/traces/sra40/SRR/003458/SRR3541303 /data/packages/galaxy/database/jobs_directory/000/21/working/sra/SRR3541303.sra.tmp.10369.tmp

```

When I tried the second process on the commandline, I got prompted for Key passphrase, which I have no idea what it was. When I just pressed enter, I got a “failed to authenticate, exiting.” message. I’ve extensively searched on google and here, but didn’t get any info about such problems. I didn’t see any info about such issues in galaxy document (of course I could have missted it). Any info is appreciaed.

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<div class="post-metadata">

**Author:** ![spz1st](https://avatars.discourse-cdn.com/v4/letter/s/8dc957/32.png) [@spz1st](https://help.galaxyproject.org/u/spz1st)\
**Post date:** [September 9, 2019, 2:13pm UTC](https://help.galaxyproject.org/t/problem-with-download-and-extract-reads-in-fasta-q-format-from-ncbi-sra-for-local-galaxy/2056/2 "2019-09-09T14:13:04Z")

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I’ve found out the cause and fixed the problem. The private key file /usr/local/bin/asperaweb\_id\_dsa.openssh didn’t exist. I downloaded the aspera connect from [https://downloads.asperasoft.com/](https://downloads.asperasoft.com/) copied the file asperaweb\_id\_dsa.openssh to /usr/local/bin/.