# Problems with the tool featureCounts: Fatal error: Exit code 255 ()

**URL:** <https://help.galaxyproject.org/t/problems-with-the-tool-featurecounts-fatal-error-exit-code-255/4837>\
**Category:** usegalaxy.org support\
**Tags:** transcriptomics, reference-annotation\
**Created:** [November 13, 2020, 3:47pm UTC](https://help.galaxyproject.org/t/problems-with-the-tool-featurecounts-fatal-error-exit-code-255/4837 "2020-11-13T15:47:10Z")\
**Posts on this page:** 1\
**Showing post:** 3

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**Author:** ![gallardoalba](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/gallardoalba/32/1903_2.png) [@gallardoalba](https://help.galaxyproject.org/u/gallardoalba)\
**Post date:** [January 9, 2021, 9:26am UTC](https://help.galaxyproject.org/t/problems-with-the-tool-featurecounts-fatal-error-exit-code-255/4837/3 "2021-01-09T09:26:00Z")

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Hi @Marcel and @q_thistle,  
the problem is that by default featureCounts set _exon_ as feature filter (Advanced options/GFF feature type filter), but this feature doesn’t appear in the _Pseudomonas_ annotation file (third column). You need to specify _CDS_ as feature type in featureCounts.

Regards

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_[View the full topic](https://help.galaxyproject.org/t/problems-with-the-tool-featurecounts-fatal-error-exit-code-255/4837)._