# Problems with the tool featureCounts: Fatal error: Exit code 255 ()

**URL:** <https://help.galaxyproject.org/t/problems-with-the-tool-featurecounts-fatal-error-exit-code-255/4837>\
**Category:** usegalaxy.org support\
**Tags:** transcriptomics, reference-annotation\
**Created:** [November 13, 2020, 3:47pm UTC](https://help.galaxyproject.org/t/problems-with-the-tool-featurecounts-fatal-error-exit-code-255/4837 "2020-11-13T15:47:10Z")\
**Posts on this page:** 1\
**Showing post:** 5

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [November 17, 2022, 12:25am UTC](https://help.galaxyproject.org/t/problems-with-the-tool-featurecounts-fatal-error-exit-code-255/4837/5 "2022-11-17T00:25:56Z")

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Hi @Mila

If adjusting the tool setting is not enough, the problem could be with one or more of the input formats. These three FAQs tend to help.

> [@Getting error while using HtSeq Count](https://help.galaxyproject.org/t/getting-error-while-using-htseq-count/9017/2):
>
> You can also review these FAQs for help addressing this and related problems that often come up together. These problems can present whether you are working in Galaxy or not.
> 
> - [#working-with-gff-gft-gtf2-gff3-reference-annotation](https://training.galaxyproject.org/training-material/faqs/galaxy/#working-with-gff-gft-gtf2-gff3-reference-annotation)
> - [#working-with-very-large-fasta-datasets](https://training.galaxyproject.org/training-material/faqs/galaxy/#working-with-very-large-fasta-datasets)
> - [#mismatched-chromosome-identifiers-and-how-to-avoid-them](https://training.galaxyproject.org/training-material/faqs/galaxy/#mismatched-chromosome-identifiers-and-how-to-avoid-them)

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