# Reference annotation GTF options for UCSC's mouse builds mm9, mm10

**URL:** <https://help.galaxyproject.org/t/reference-annotation-gtf-options-for-ucscs-mouse-builds-mm9-mm10/5173>\
**Category:** usegalaxy.eu support\
**Tags:** transcriptomics, reference-annotation\
**Created:** [January 11, 2021, 1:03pm UTC](https://help.galaxyproject.org/t/reference-annotation-gtf-options-for-ucscs-mouse-builds-mm9-mm10/5173 "2021-01-11T13:03:56Z")\
**Posts on this page:** 1\
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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [January 11, 2021, 9:53pm UTC](https://help.galaxyproject.org/t/reference-annotation-gtf-options-for-ucscs-mouse-builds-mm9-mm10/5173/2 "2021-01-11T21:53:16Z")

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Hello @Gaia_Gentile

Gencode annotation for the mouse builds mm9 and mm10 are available from the Gencode website.

Instructions for how to load and prepare that annotation for use with tools is covered in this prior Q&A. It is about a specific tool and the human genome, but the same advice applies:

- [RNA-STAR and hg38 GTF reference annotation](https://help.galaxyproject.org/t/rna-star-and-hg38-gtf-reference-annotation/749)

You may also find this FAQ helpful:

- [Extended Help for Differential Expression Analysis Tools](https://galaxyproject.org/support/diff-expression/)

I also added a few tags to your post that will lead to more related topics. Or, review the results of this search:

- [Search results for 'reference gtf' - Galaxy Community Help](https://help.galaxyproject.org/search?q=reference%20gtf)

If that doesn’t answer you question, please explain more about the steps you have done so far (tools), and which data is involved (reference genome, reference annotation). If you are following a tutorial, include a link to that too please.

Thanks!

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_[View the full topic](https://help.galaxyproject.org/t/reference-annotation-gtf-options-for-ucscs-mouse-builds-mm9-mm10/5173)._
