# Reference based genome assembly of Bacterial Illumina reads tutorial in Galaxy

**URL:** <https://help.galaxyproject.org/t/reference-based-genome-assembly-of-bacterial-illumina-reads-tutorial-in-galaxy/11785>\
**Category:** Uncategorized\
**Tags:** gtn-tutorial\
**Created:** [February 26, 2024, 4:31am UTC](https://help.galaxyproject.org/t/reference-based-genome-assembly-of-bacterial-illumina-reads-tutorial-in-galaxy/11785 "2024-02-26T04:31:00Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![animeshsen92](https://avatars.discourse-cdn.com/v4/letter/a/3ec8ea/32.png) [@animeshsen92](https://help.galaxyproject.org/u/animeshsen92)\
**Post date:** [February 26, 2024, 4:31am UTC](https://help.galaxyproject.org/t/reference-based-genome-assembly-of-bacterial-illumina-reads-tutorial-in-galaxy/11785/1 "2024-02-26T04:31:00Z")

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I unable to perform Reference based genome Assembly in Galaxy. Kindly Guide me or provide me any tutorial link regarding this. Thank You.

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**Author:** ![igor](https://avatars.discourse-cdn.com/v4/letter/i/dec6dc/32.png) [@igor](https://help.galaxyproject.org/u/igor)\
**Post date:** [February 26, 2024, 5:22am UTC](https://help.galaxyproject.org/t/reference-based-genome-assembly-of-bacterial-illumina-reads-tutorial-in-galaxy/11785/2 "2024-02-26T05:22:26Z")

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Hi @animeshsen92  
Assembly section at Galaxy Training Network: [Assembly / Tutorial List](https://training.galaxyproject.org/training-material/topics/assembly/)

What do you mean by reference based assembly? Is it variant calling? If yes, check Variant Analysis section in Galaxy Training Network. If you after comparison between different strains, consider snippy followed by snippy-core. For some projects variant calling might be more advantageous than genome assembly.

I have impression that in these days people rarely do reference guided assembly of bacterial genomes.

Kind regards,  
Igor