Reference genes for qPCR

Hello, I am analyzing sequences obtained from leukemic cells and I am comparing them with hematopoietic stem cells, but the most used reference genes show a lot of variation … there is a Galaxy tool that allows me to determine which or these genes are the most suitable …

Hi @Alejandra1819,
I suggest you perform a differential expression analysis (e.g. by using DESeq2), filtering those genes whose fold change is near to zero by using the Filter data on any column tool and sort the output by descending order based on the Standart Error. Then you can select as the reference one of the genes whose mean expression is significant, and with low dispersion.


Hello @gallardoalaba, I thank you for your suggestion, it was very useful to me.

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