# regarding sequence assembly **URL:** \ **Category:** Uncategorized\ **Created:** [January 1, 2021, 6:52am UTC](https://help.galaxyproject.org/t/regarding-sequence-assembly/5110 "2021-01-01T06:52:23Z")\ **Posts on this page:** 1\ **Showing post:** 2
**Author:** ![gallardoalba](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/gallardoalba/32/1903_2.png) [@gallardoalba](https://help.galaxyproject.org/u/gallardoalba)\ **Post date:** [January 1, 2021, 10:29am UTC](https://help.galaxyproject.org/t/regarding-sequence-assembly/5110/2 "2021-01-01T10:29:43Z")
HI @Anand_Mishra, firstly in order to perform the transcriptome assembly, it is necessary to select the samples corresponding to a specific specie; since the _SRX5718892_ includes transcriptomic data from two different _Nepeta species_, it is necessary to select a [subset of sequences](https://www.ncbi.nlm.nih.gov/Traces/study/?uids=7701807%2C7701805%2C7701804%2C7701803%2C7701802%2C7701801%2C7701800&o=acc_s%3Aa&s=SRR8938416,SRR8938418,SRR8938413,SRR8938417,SRR8938420,SRR8938415,SRR8938419) (e.g. _Nepeta cataria_ samples). From this database entry, you can download the Accession List file. The next step is to upload that file to Galaxy by using the **Uploader tool**. Finally, you can use the **Faster Download and Extract Reads in FASTQ** format from the NCBI SRA tool to download the FASTQ datasets. You can find a detailed explanation about how to perform the transcriptome assembly in the following link. > [@de novo assembly using Trinity in Galaxy](https://help.galaxyproject.org/t/de-novo-assembly-using-trinity-in-galaxy/2760): > > Hi I’m studying on a plant that has no reference genome and only has one scaffold assembly and one gff3 annotation file. If I want to do de novo assembly using the Galaxy Can I import the links of 20 fastq files from the RNA-seq of the samples together to construct the Reference Genome with Trinity ? If this is possible, please help me import the links of these files together into Galaxy and make the reference genome. Maximum number of samples or data can be used for Deno Assembly in … Regards --- _[View the full topic](https://help.galaxyproject.org/t/regarding-sequence-assembly/5110)._