regd fastqc ouput

Hi,
In untrimmed fast QC it shows me errors :
Per sequence GC content = !
Sequence Length Distribution = !
Sequence Duplication Levels = !
So how i can the improve the duplication level ??
This one is before trimming:

This one is after trrimming:

Hi @Thara_Dharanidhar,
I suggest you to remove the first 10 nucleotides form the 5’ end by using PRINSEQ.

Regards

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