# RNA STAR trouble shooting error in samples

**URL:** https://help.galaxyproject.org/t/rna-star-trouble-shooting-error-in-samples/6713
**Category:** usegalaxy.org support
**Tags:** transcriptomics, rna\_star
**Created:** [September 24, 2021, 1:02pm UTC](https://help.galaxyproject.org/t/rna-star-trouble-shooting-error-in-samples/6713 "2021-09-24T13:02:48Z")
**Posts on this page:** 1
**Page:** 1

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### Author: ![Gould\_r](https://avatars.discourse-cdn.com/v4/letter/g/7cd45c/32.png) [@Gould\_r](https://help.galaxyproject.org/u/Gould_r)
#### Post date: [September 24, 2021, 1:02pm UTC](https://help.galaxyproject.org/t/rna-star-trouble-shooting-error-in-samples/6713/1 "2021-09-24T13:02:48Z")

</div>

Hi all. New to Galaxy and have been using RNA STAR for mapping 6 samples. 5/6 samples mapped with no errors, giving logs, splice junction and mapped files.  
I’ve inputed the samples via the server and the cloud; and also uploaded a specific reference genome using Ensembl. Fastqc and Multiqc functioned fine across all 6 samples using galaxy.  
However, 1 sample is not processing using RNA STAR, including on re-running the job and checking the input parameters.  
The job is returning: Tool Standard Error (33, “HTTP server doesn’t seem to support byte ranges. Cannot resume.”)

 ![image](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/e/ec475d73568909b59dd871586ffb85ce63dc8edd.png)  
 ![image](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/1/19c8868d9c08eda404c355d24032927838d18247.png)  
 ![image](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/4/40440856ea195325b19f2da0882a1d0dd0b1f31e.png)

Would massively appreciate some help.