RNA STARSolo longer barcode length

Hello Galaxy team,

I am using RNA STARSolo from Galaxy which is really useful tool for people who are not familiar with the bioinformatics. I was using tutorial from the following link - Pre-processing of 10X Single-Cell RNA Datasets
Most of time, it works great. However, some of our and others dataset has R1 barcode length that are 151bp long (R2 is also 151bp), and I was wondering if there is any way to do pre-processing of those data. Help will be really appreciated.

Thank you,


Dear @Young,
For a different bacode-(length) you can check out the option Configure Chemistry Options and pick Custom, then you can and have to define the barcode details yourself.

Kind regards,

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