# RNAstar not working after two days

**URL:** <https://help.galaxyproject.org/t/rnastar-not-working-after-two-days/16031>\
**Category:** usegalaxy.org support\
**Tags:** rna\_star\
**Created:** [July 25, 2025, 8:33am UTC](https://help.galaxyproject.org/t/rnastar-not-working-after-two-days/16031 "2025-07-25T08:33:54Z")\
**Posts on this page:** 4\
**Page:** 1

<div class="post-metadata">

**Author:** ![ChloeB1999](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/chloeb1999/32/7498_2.png) [@ChloeB1999](https://help.galaxyproject.org/u/ChloeB1999)\
**Post date:** [July 25, 2025, 8:33am UTC](https://help.galaxyproject.org/t/rnastar-not-working-after-two-days/16031/1 "2025-07-25T08:33:54Z")

</div>

Hi,

I am trying to run RNAstar onm my datasets and it completed 3 almost straight away and hasn’t progressed any further for 2 days. When I ran it on near identical datasets last week it did it straight away. I am using the [https://usegalaxy.org](https://usegalaxy.org) server and my username is chloe1999

 ![image](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/c/c1cf34894c01837019da3a36bb5606d726f59872.png)

---

<div class="post-metadata">

**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [July 25, 2025, 5:32pm UTC](https://help.galaxyproject.org/t/rnastar-not-working-after-two-days/16031/2 "2025-07-25T17:32:09Z")

</div>

Welcome @ChloeB1999

Your jobs are most likely queued. This is expected at any time but especially right now. With the [UseGalaxy.eu server offline for maintenance](https://help.galaxyproject.org/t/galaxy-europe-maintenance-48-hrs-downtime-between-18-30-cest-on-fri-july-25-and-19-00-cest-on-sun-july-27/16021) there are likely many more people working at the [UseGalaxy.org](http://UseGalaxy.org) server.

So, if you inputs are ready to process and the mapping tool is queued, you’ll need to wait for that to complete. Avoid stopping or restarting, since that will put all of your new jobs back at the end of the job queue again.

If you want to share back workflow invocation we can take a look to make sure more is not going on. Sharing the invocation is like sharing a history but happens in fewer clicks. Maybe give that a try? You can paste the link back here in a reply.

_Screenshot_

 ![share-invocation](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/2/25049fc943760be6a2ac0ad12e3fae01fdda22ba.png)

**XRef**

> [@Troubleshooting resources for errors or unexpected results](https://help.galaxyproject.org/t/troubleshooting-resources-for-errors-or-unexpected-results/42/1):
>
> When working at [UseGalaxy.org](http://UseGalaxy.org), [UseGalaxy.eu](http://UseGalaxy.eu), or [UseGalaxy.org.au](http://UseGalaxy.org.au), your analysis jobs execute [at large scale academic clusters](https://training.galaxyproject.org/training-material/faqs/galaxy/#analysis). Hundreds of thousands of jobs process each month!
> 
> - Use a [Workflow](https://training.galaxyproject.org/training-material/search2?query=workflow) for batch work.
> - Allow [queued (grey color) and executing (yellow-orange) datasets](https://training.galaxyproject.org/training-material/faqs/galaxy/datasets_job_status.html) to finish processing.
> - Deleting and rerunning puts your job back _at the end_ of the queue again!
> - Job priority is the exactly the same for everyone.
> - **Be patient**. Galaxy is a free service, when a lot of people are using it, [you may have to wait longer than usual](https://training.galaxyproject.org/training-material/faqs/galaxy/analysis_jobs_not_running.html) (especially for ‘big’ jobs, e.g. alignments).

* * *

Let’s start there! 🙂

---

<div class="post-metadata">

**Author:** ![ChloeB1999](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/chloeb1999/32/7498_2.png) [@ChloeB1999](https://help.galaxyproject.org/u/ChloeB1999)\
**Post date:** [July 25, 2025, 6:52pm UTC](https://help.galaxyproject.org/t/rnastar-not-working-after-two-days/16031/3 "2025-07-25T18:52:34Z")

</div>

Hi @jennaj

Thank you for replying, heres my invocations, hopefully it is just queued,

> **[Galaxy](https://usegalaxy.org/workflows/invocations/b4a642435a3b6166)**
>
> Galaxy Workflows facilitate rigorous, reproducible analysis pipelines that can be shared with the community.

---

<div class="post-metadata">

**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [July 25, 2025, 8:39pm UTC](https://help.galaxyproject.org/t/rnastar-not-working-after-two-days/16031/4 "2025-07-25T20:39:48Z")

</div>

Thanks for sharing the history @ChloeB1999

Everything looks fine to me, and the jobs appear to be queued. I’ve asked internally for confirmation. I’ll update here if anything odd is discovered. So – try leaving the jobs queued. 🧑‍🔬
