# run Unicycler always got " unicycler: command not found" -- RESOLVED at UseGalaxy.org 20230210 **URL:** \ **Category:** usegalaxy.org support\ **Tags:** troubleshooting, fastqsanger\ **Created:** [January 16, 2023, 5:25pm UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297 "2023-01-16T17:25:57Z")\ **Posts on this page:** 12\ **Page:** 1
**Author:** ![Meadowlawn](https://avatars.discourse-cdn.com/v4/letter/m/34f0e0/32.png) [@Meadowlawn](https://help.galaxyproject.org/u/Meadowlawn)\ **Post date:** [January 16, 2023, 5:25pm UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297/1 "2023-01-16T17:25:57Z")
run Unicycler always got “/jetstream2/scratch/main/jobs/47842391/command.sh: line 110: unicycler: command not found” I am wondering if there are something wrong with my input data or there is something wrong with the Unicycler? Thanks. ---
**Author:** ![igor](https://avatars.discourse-cdn.com/v4/letter/i/dec6dc/32.png) [@igor](https://help.galaxyproject.org/u/igor)\ **Post date:** [January 17, 2023, 12:46am UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297/2 "2023-01-17T00:46:19Z")
Hi @Meadowlawn I reproduced the issue with Unicycler. I hope it will be fixed soon. @jennaj It seems there is an issue with Unicycler on [usegalaxy.org](http://usegalaxy.org). Kind regards, Igor ---
**Author:** ![Meadowlawn](https://avatars.discourse-cdn.com/v4/letter/m/34f0e0/32.png) [@Meadowlawn](https://help.galaxyproject.org/u/Meadowlawn)\ **Post date:** [January 17, 2023, 3:20pm UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297/3 "2023-01-17T15:20:14Z")
Hi Igor, Thank you very much for quick reply. I tired today and found it still does not work. Current error is “line 10: unicycler: command not found”. The error changed from “line 110 command not found” to “line 10 command not found” Thanks again, Guowu ---
**Author:** ![igor](https://avatars.discourse-cdn.com/v4/letter/i/dec6dc/32.png) [@igor](https://help.galaxyproject.org/u/igor)\ **Post date:** [January 17, 2023, 11:21pm UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297/4 "2023-01-17T23:21:23Z")
Hi Guowu, if you need results urgently, maybe copy FASTQ files to another Galaxy server, such as [usegalaxy.eu](http://usegalaxy.eu), and complete assembly over there. It is OK to have accounts on several public Galaxy servers. To copy a file, click on dataset name in history on ‘source’ Galaxy, click at Copy Link (chain icon), on ‘target’ Galaxy activate Upload menu, switch to Paste/Fetch Data tub, paste the link and hit Start. Kind regards, Igor ---
**Author:** ![Meadowlawn](https://avatars.discourse-cdn.com/v4/letter/m/34f0e0/32.png) [@Meadowlawn](https://help.galaxyproject.org/u/Meadowlawn)\ **Post date:** [January 18, 2023, 2:23pm UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297/5 "2023-01-18T14:23:48Z")
Hi Igor, Thank you for the instructions. I will try it. When do you think it will be fixed? Thanks again, Guowu ---
**Author:** ![igor](https://avatars.discourse-cdn.com/v4/letter/i/dec6dc/32.png) [@igor](https://help.galaxyproject.org/u/igor)\ **Post date:** [January 19, 2023, 5:17am UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297/6 "2023-01-19T05:17:03Z")
Hi Guowu, unfortunately, I don’t know, but I copied my reply to Jenifer. Kind regards, Igor ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [January 19, 2023, 9:01pm UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297/7 "2023-01-19T21:01:03Z")
**Update** Jan 24 2022 All test runs for the three most current versions of Unicycler were successful. I’m going to consider this issue resolved/closed unless a bug report for a job run from now forward is sent in. If doing that, comment here and include a link to this topic in the bug report comments to help find it. Public test history based on tutorial data: [test-history-unicycler](https://usegalaxy.org/u/jen-galaxyproject/h/test-history-unicycler) * * * Hi @igor I started up some tests but had to restart again. Still running now. @Meadowlawn – would you please 1. try a rerun now in a clean history 2. be sure to use the most current version of the tool 3. if you have not done QA steps on the reads, do that first, then assemble 4. if the job still fails, please submit a bug report from that history and the errored run. It would be helpful if you posted in the URL for this question into the comments field. **Unicycler** requires uncompressed intact pairs of short reads (Illumina) and optionally long reads (ONT, etc). If the tool cannot read the inputs, it can error with the message you received. Tutorials - [Quality Control](https://training.galaxyproject.org/training-material/topics/sequence-analysis/tutorials/quality-control/tutorial.html) - [Unicycler Assembly](https://training.galaxyproject.org/training-material/topics/assembly/tutorials/unicycler-assembly/tutorial.html) ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [January 24, 2023, 6:24pm UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297/8 "2023-01-24T18:24:42Z")
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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [January 24, 2023, 6:25pm UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297/9 "2023-01-24T18:25:54Z")
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**Author:** ![igor](https://avatars.discourse-cdn.com/v4/letter/i/dec6dc/32.png) [@igor](https://help.galaxyproject.org/u/igor)\ **Post date:** [January 26, 2023, 11:05pm UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297/10 "2023-01-26T23:05:18Z")
Hi @jennaj I am still getting errors with Unicycler on the main Galaxy server; the last on was on January 25th: > **[Galaxy](https://usegalaxy.org/published/history?id=6e67fca883e321d4)** > > Galaxy is a community-driven web-based analysis platform for life science research. I don’t have problems with the same datasets, both fastqsanger and compressed versions, in Europe. The same version of Unicycler was used with the default settings: [https://usegalaxy.eu/u/igor/h/unicycler-test](https://usegalaxy.eu/u/igor/h/unicycler-test) I know I am absent-minded, but I could not figure out what I am doing wrong: select Unicycler, select input files, click Execute or Run Tool. The only difference I found is in Galaxy versions: the main server has 23.0.rc1, Europe uses 22.05. I have a normal user account on the main server and in Europe. Any chance it works for admin accounts only? Kind regards, Igor ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [February 10, 2023, 8:05pm UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297/11 "2023-02-10T20:05:59Z")
Thanks @igor We did some more adjustments this morning at [UseGalaxy.org](http://UseGalaxy.org). All tests were successful 🥳 Anyone that had a recent failure with this tool should please try again now. ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [February 10, 2023, 8:08pm UTC](https://help.galaxyproject.org/t/run-unicycler-always-got-unicycler-command-not-found-resolved-at-usegalaxy-org-20230210/9297/12 "2023-02-10T20:08:53Z")