# Running 4 lefse analysis at the same time in order to save space for graphic

**URL:** <https://help.galaxyproject.org/t/running-4-lefse-analysis-at-the-same-time-in-order-to-save-space-for-graphic/4838>\
**Category:** usegalaxy.org support\
**Tags:** lefse\
**Created:** [November 13, 2020, 6:15pm UTC](https://help.galaxyproject.org/t/running-4-lefse-analysis-at-the-same-time-in-order-to-save-space-for-graphic/4838 "2020-11-13T18:15:32Z")\
**Posts on this page:** 3\
**Page:** 1

<div class="post-metadata">

**Author:** ![VinceBilly](https://avatars.discourse-cdn.com/v4/letter/v/5f9b8f/32.png) [@VinceBilly](https://help.galaxyproject.org/u/VinceBilly)\
**Post date:** [November 13, 2020, 6:15pm UTC](https://help.galaxyproject.org/t/running-4-lefse-analysis-at-the-same-time-in-order-to-save-space-for-graphic/4838/1 "2020-11-13T18:15:32Z")

</div>

Dear all,

I am comparing microbial community in response to a treatment using lefse cladogram.  
I have 4 different experimental set up and I am planning to display the 4 lefse analysis together in order to compare result between experiment (see figure attached, "CURRENT GRAPH). So far I have run 4 lefse analyses separately but I am realising that it makes the reading of the graph difficult, cause I have 4 different legend/letters (see figure attached, "CURRENT GRAPH). Is it possible to run 4 lefse analysis separately (from 4 distincts experiments) from the same table input, and therefore get one unique taxa legend for all 4 cladograms. I would like to get the result bellow (see figure attached, “GRAPH EXPECTED”).

Thanks very much for your help.

 ![Screenshot 2020-11-13 at 10.14.41](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/8/8dde5062591369ec3ff4b2302cef3d32a8c247dc.png)

---

<div class="post-metadata">

**Author:** ![David](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/david/32/7031_2.png) [@David](https://help.galaxyproject.org/u/David)\
**Post date:** [January 21, 2021, 12:31am UTC](https://help.galaxyproject.org/t/running-4-lefse-analysis-at-the-same-time-in-order-to-save-space-for-graphic/4838/2 "2021-01-21T00:31:41Z")

</div>

In this case you are assuming that a given taxa color (up/down difference abundance) is the same in all the 4 cladograms. I wonder if that would be rare to happen.  
But  
You could simply “merge” all the legend text (and the colored rectangles) in a graphics editor like inkscape, gimp, photoshop, etc. This is what I used to do. But you need to confirm what I said above.  
Good luck

---

<div class="post-metadata">

**Author:** ![VinceBilly](https://avatars.discourse-cdn.com/v4/letter/v/5f9b8f/32.png) [@VinceBilly](https://help.galaxyproject.org/u/VinceBilly)\
**Post date:** [May 5, 2021, 7:54pm UTC](https://help.galaxyproject.org/t/running-4-lefse-analysis-at-the-same-time-in-order-to-save-space-for-graphic/4838/3 "2021-05-05T19:54:00Z")

</div>

Right, it is unlikely that difference of taxa between plots would be the same.  
Good idea for inkscape  
Thanks