# RUVSeq Error 'Fatal error: An undefined error occurred, please check your input carefully and contact your administrator.'

**URL:** <https://help.galaxyproject.org/t/ruvseq-error-fatal-error-an-undefined-error-occurred-please-check-your-input-carefully-and-contact-your-administrator/7972>\
**Category:** usegalaxy.org support\
**Tags:** troubleshooting, salmon\
**Created:** [May 3, 2022, 3:03pm UTC](https://help.galaxyproject.org/t/ruvseq-error-fatal-error-an-undefined-error-occurred-please-check-your-input-carefully-and-contact-your-administrator/7972 "2022-05-03T15:03:52Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Edward\_Chen](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/edward_chen/32/2454_2.png) [@Edward\_Chen](https://help.galaxyproject.org/u/Edward_Chen)\
**Post date:** [May 3, 2022, 3:03pm UTC](https://help.galaxyproject.org/t/ruvseq-error-fatal-error-an-undefined-error-occurred-please-check-your-input-carefully-and-contact-your-administrator/7972/1 "2022-05-03T15:03:52Z")

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Hi,

I’m pretty sure I glossed over something and therefore caused an issue, but I’ve been trying to use RUVSeq ( **Remove Unwanted Variation** from RNA-seq data (Galaxy Version 1.26.0+galaxy0)) through inputting Salmon files. I have selected the corresponding files for each factor and made sure to select the Salmon option in the settings. I do believe that the error has something to do with the following:

Error in read.table(tx2gene, header = has\_header) :  
more columns than column names  
Calls: get\_deseq\_dataset → read.table

Thanks for the help.

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [December 21, 2023, 8:39pm UTC](https://help.galaxyproject.org/t/ruvseq-error-fatal-error-an-undefined-error-occurred-please-check-your-input-carefully-and-contact-your-administrator/7972/2 "2023-12-21T20:39:15Z")

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