# SICER is not available for the genome.

**URL:** <https://help.galaxyproject.org/t/sicer-is-not-available-for-the-genome/13218>\
**Category:** Uncategorized\
**Tags:** reference-index, custom-genome, reference-genome, custom-build, tool-help, peakcalling\_sicer\
**Created:** [August 13, 2024, 2:08pm UTC](https://help.galaxyproject.org/t/sicer-is-not-available-for-the-genome/13218 "2024-08-13T14:08:33Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![moonmoondeb](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/moonmoondeb/32/5473_2.png) [@moonmoondeb](https://help.galaxyproject.org/u/moonmoondeb)\
**Post date:** [August 13, 2024, 2:08pm UTC](https://help.galaxyproject.org/t/sicer-is-not-available-for-the-genome/13218/1 "2024-08-13T14:08:33Z")

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Hi  
I want to use the SICER tool for my histone chip-set analysis. But its showing “SICER is not available for the genome.”  
I am using Chicken genome assembly GRCg7b. (NCBI RefSeq assembly  
GCF\_016699485.2 and Submitted GenBank assembly  
GCA\_016699485.1).  
If this genome assembly is not available for the tool, how can I make it available.  
Kindly help me.  
With regards  
Moonmoon

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<div class="post-metadata">

**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [August 13, 2024, 6:59pm UTC](https://help.galaxyproject.org/t/sicer-is-not-available-for-the-genome/13218/2 "2024-08-13T18:59:17Z")

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Hello @moonmoondeb

For how to include your own genome for use with tools, please see the guides below.

> [@Custom genome + custom build: How to use a genome that is not natively indexed at the server you are working at](https://help.galaxyproject.org/t/custom-genome-custom-build-how-to-use-a-genome-that-is-not-natively-indexed-at-the-server-you-are-working-at/10082/2):
>
> Try using the **custom genome** feature.
> 
> [FAQ: How to use Custom Reference Genomes?](https://training.galaxyproject.org/training-material/faqs/galaxy/reference_genomes_custom_genomes.html)
> 
> If you need to assign a **database** metadata to datasets, your custom genome can be promoted to a **custom build**.
> 
> [FAQ: Adding a custom database/build (dbkey)](https://training.galaxyproject.org/training-material/faqs/galaxy/analysis_add_custom_build.html)

* * *

With that context, to answer your question here

> [@moonmoondeb](#):
>
> I want to use the SICER tool for my histone chip-set analysis. But its showing “SICER is not available for the genome.”  
> I am using Chicken genome assembly GRCg7b. (NCBI RefSeq assembly  
> GCF\_016699485.2 and Submitted GenBank assembly  
> GCA\_016699485.1).  
> If this genome assembly is not available for the tool, how can I make it available.

The solution would be to create a **Custom Build** database key for your genome, and to assign that to your input datasets. This will allow the tool to access the fasta index for your genome (to interpret scientific options like **Effective genome fraction** ).

_Be sure to carefully read that guide_: format your genome fasta in a simple way, and create a unique database key that is all **oneWord** with no special characters, as an example **GCA016699485** should work fine.

Do not reuse any _existing_ server database key or expect problems.

* * *

For related topics here at this forum, including more help with troubleshooting how these work, please see #custom-genome and #custom-build .

Any questions you may have, please ask and we’ll try to help more. 🙂