# snpsift annotate for annotating gnomad and dbsnp ids

**URL:** <https://help.galaxyproject.org/t/snpsift-annotate-for-annotating-gnomad-and-dbsnp-ids/2340>\
**Category:** Uncategorized\
**Created:** [October 23, 2019, 7:33am UTC](https://help.galaxyproject.org/t/snpsift-annotate-for-annotating-gnomad-and-dbsnp-ids/2340 "2019-10-23T07:33:06Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![roselucia](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/roselucia/32/986_2.png) [@roselucia](https://help.galaxyproject.org/u/roselucia)\
**Post date:** [October 23, 2019, 7:33am UTC](https://help.galaxyproject.org/t/snpsift-annotate-for-annotating-gnomad-and-dbsnp-ids/2340/1 "2019-10-23T07:33:06Z")

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Dear all,  
after I annotated my vcfs with snpeff, I would know like to annotate further information as gnomad frequencies and dbsnp ids using snpsift. Unfortunatly trying to annotate gnomad\_exome frequencies for one chromosme (chr17) leaves me with an error message.  
I loaded the according vcf from gnomad on my galaxy docker via URL ([https://storage.googleapis.com/gnomad-public/release/2.1.1/vcf/exomes/gnomad.exomes.r2.1.1.sites.17.vcf.bgz](https://storage.googleapis.com/gnomad-public/release/2.1.1/vcf/exomes/gnomad.exomes.r2.1.1.sites.17.vcf.bgz)), changed the datatype into .vcf (using the pencil icon). However I get this error message when using snpsift annotate: “Unable to finish job”

 ![36](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/f/fac6605675a782f3e156a4f9638ecd7a5ca05822.png) ![26](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/7/7fb332b7cb1756d9f407e220173847e92a1f26eb.png)

Thanks for the help!  
Rose

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**Author:** ![roselucia](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/roselucia/32/986_2.png) [@roselucia](https://help.galaxyproject.org/u/roselucia)\
**Post date:** [October 23, 2019, 9:31am UTC](https://help.galaxyproject.org/t/snpsift-annotate-for-annotating-gnomad-and-dbsnp-ids/2340/2 "2019-10-23T09:31:40Z")

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I tried it as well on use [galaxy.org](http://galaxy.org) and was left with the following error message:  
Picked up \_JAVA\_OPTIONS: -Djava.io.tmpdir=/galaxy-repl/main/jobdir/025/479/25479116/\_job\_tmp -Xmx7g -Xms256m  
VcfFileIterator.parseVcfLine(132): Fatal error reading file ‘null’ (line: 1):  
��BCX+�}}s�8��߹O�ڭڛ��i��%^͕"K�n-ٱ���mM�d���#�QJ�}�|�k���(Ң,;

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**Author:** ![roselucia](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/roselucia/32/986_2.png) [@roselucia](https://help.galaxyproject.org/u/roselucia)\
**Post date:** [October 28, 2019, 10:08am UTC](https://help.galaxyproject.org/t/snpsift-annotate-for-annotating-gnomad-and-dbsnp-ids/2340/3 "2019-10-28T10:08:42Z")

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> [@roselucia](#):
>
> using

@jennaj May I ask you for help in this regard?

Thanks a lot