RNA-STAR, hg38 GTF reference annotation, Cloudman/AWS options plus local Galaxy "Cloud Bursting" for memory intensive mapping

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Hi @jgoldst7

Jobs can error for all sort of odd reasons (including memory reasons) when there are problems with the inputs.

In your case, I suspect the reference annotation is a mismatch for the reference genome. The built-in “hg38” human genome is sourced from UCSC. Please see this prior Q&A for more help and details: RNA-STAR and hg38 GTF reference annotation

FAQ: https://galaxyproject.org/support/

Thanks!