# tools that require bigwig input cannot use bedgraph (as bigwig) file

**URL:** <https://help.galaxyproject.org/t/tools-that-require-bigwig-input-cannot-use-bedgraph-as-bigwig-file/11400>\
**Category:** Uncategorized\
**Tags:** troubleshooting\
**Created:** [January 3, 2024, 3:57pm UTC](https://help.galaxyproject.org/t/tools-that-require-bigwig-input-cannot-use-bedgraph-as-bigwig-file/11400 "2024-01-03T15:57:34Z")\
**Posts on this page:** 1\
**Showing post:** 15

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [July 9, 2024, 9:00pm UTC](https://help.galaxyproject.org/t/tools-that-require-bigwig-input-cannot-use-bedgraph-as-bigwig-file/11400/15 "2024-07-09T21:00:57Z")

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Hi @TTP

I see both tools at [UseGalaxy.org](http://UseGalaxy.org) today:

One with the option for trimming overhanging coordinates (the version that is best to use after MACS2) → [https://usegalaxy.org/root?tool\_id=toolshed.g2.bx.psu.edu/repos/iuc/ucsc\_wigtobigwig/ucsc\_wigtobigwig/447+galaxy0](https://usegalaxy.org/root?tool_id=toolshed.g2.bx.psu.edu/repos/iuc/ucsc_wigtobigwig/ucsc_wigtobigwig/447+galaxy0)

And one with the direct conversion (can produce errors with sensitive downstream tools, if the data is from MACS2) → [https://usegalaxy.org/root?tool\_id=CONVERTER\_bedgraph\_to\_bigwig](https://usegalaxy.org/root?tool_id=CONVERTER_bedgraph_to_bigwig)

And for the error about the memory allocation – I can’t remember if you sent something in? Did you include a link to this topic? That will help me to associate the two. If you want to send that in again, I’ll review again 🙂

Hope this helps!

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_[View the full topic](https://help.galaxyproject.org/t/tools-that-require-bigwig-input-cannot-use-bedgraph-as-bigwig-file/11400)._
