# Unable to select VCF with Gemini load? Tool form includes full input requirements

**URL:** <https://help.galaxyproject.org/t/unable-to-select-vcf-with-gemini-load-tool-form-includes-full-input-requirements/8207>\
**Category:** Uncategorized\
**Tags:** metadata, troubleshooting, variant-analysis, vcf, snpeff\
**Created:** [June 16, 2022, 9:25pm UTC](https://help.galaxyproject.org/t/unable-to-select-vcf-with-gemini-load-tool-form-includes-full-input-requirements/8207 "2022-06-16T21:25:15Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Shuchi](https://avatars.discourse-cdn.com/v4/letter/s/7feea3/32.png) [@Shuchi](https://help.galaxyproject.org/u/Shuchi)\
**Post date:** [June 16, 2022, 9:25pm UTC](https://help.galaxyproject.org/t/unable-to-select-vcf-with-gemini-load-tool-form-includes-full-input-requirements/8207/1 "2022-06-16T21:25:15Z")

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Hi,  
I have generated the file using snpsift annotate (snpEff tool). This file is not recognised by Gemini load. Possibly, there is an issued in type of input files.

What should be the input fie in the 2nd option?

1.Variant input file in VCF format

2.View dataEdit attributesDelete  
VCF File with ID field annotated (e.g. dbSNP.vcf)

need guidance here. Please…  
Thank you.

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [June 21, 2022, 2:10am UTC](https://help.galaxyproject.org/t/unable-to-select-vcf-with-gemini-load-tool-form-includes-full-input-requirements/8207/2 "2022-06-21T02:10:40Z")

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Hi @Shuchi

The tool is available at [UseGalaxy.eu](http://UseGalaxy.eu) and [UseGalaxy.org.au](http://UseGalaxy.org.au).

As noted on the tool form, any `vcf` dataset is accepted as an input but it must meet both of these criteria:

1. Be based on the Human GRCh37 (hg19) genome build assembly
2. Have `hg19` assigned as the database metadata attribute.

 ![gemini-vcf-input](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/f/f27ee0962db79e5485aa05a9b53aa6e9720c83a9.png)

FAQs: [Galaxy Training!](https://training.galaxyproject.org/training-material/faqs/galaxy/#analysis)

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<div class="post-metadata">

**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [April 7, 2026, 9:55pm UTC](https://help.galaxyproject.org/t/unable-to-select-vcf-with-gemini-load-tool-form-includes-full-input-requirements/8207/3 "2026-04-07T21:55:38Z")

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