# Unicycler doesn't combine two datasets

**URL:** https://help.galaxyproject.org/t/unicycler-doesnt-combine-two-datasets/9266
**Category:** usegalaxy.eu support
**Tags:** assembly, troubleshooting, fastqsanger, single-cell
**Created:** [January 10, 2023, 8:19am UTC](https://help.galaxyproject.org/t/unicycler-doesnt-combine-two-datasets/9266 "2023-01-10T08:19:37Z")
**Posts on this page:** 1
**Showing post:** 2

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### Author: ![igor](https://avatars.discourse-cdn.com/v4/letter/i/dec6dc/32.png) [@igor](https://help.galaxyproject.org/u/igor)
#### Post date: [January 10, 2023, 11:20pm UTC](https://help.galaxyproject.org/t/unicycler-doesnt-combine-two-datasets/9266/2 "2023-01-10T23:20:10Z")

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Hi @Manuel  
similar error was discussed on the forum, eg

> [@Unicycler - hybrid assembly failure](https://help.galaxyproject.org/t/unicycler-hybrid-assembly-failure/1366):
>
> Dear All, I’ve recently encountered some issues with Unicycler assembly. I’ve tried to perform hybrid assembly with use of trimmed Illumina reads R1 (0.17 Gb) + R2 (0.16 Gb); format: fastqsanger.gz nanopore reads (2.3 Gb); format: fasqsanger Unicycler readily deals with individual assembly of either Illumina or Nanopore reads. However, it fails to generate hybrid assembly. Any suggestions? maybe is it about available working memory @ Galaxy server? thanks in advance, Piotr PS here …

Hope this helps.  
Kind regards,  
Igor

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