# Usegalaxy.eu HUMAnN 3.9+galaxy1 fails because ChocoPhlAn directory contains mixed database versions

**URL:** <https://help.galaxyproject.org/t/usegalaxy-eu-humann-3-9-galaxy1-fails-because-chocophlan-directory-contains-mixed-database-versions/18339>\
**Category:** usegalaxy.eu support\
**Tags:** troubleshooting\
**Created:** [August 25, 2026, 12:52am UTC](https://help.galaxyproject.org/t/usegalaxy-eu-humann-3-9-galaxy1-fails-because-chocophlan-directory-contains-mixed-database-versions/18339 "2026-08-25T00:52:12Z")\
**Posts on this page:** 1\
**Showing post:** 2

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [August 25, 2026, 3:29am UTC](https://help.galaxyproject.org/t/usegalaxy-eu-humann-3-9-galaxy1-fails-because-chocophlan-directory-contains-mixed-database-versions/18339/2 "2026-08-25T03:29:16Z")

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> [@HUMAnN fails due to MetaPhlAn database mismatch](https://help.galaxyproject.org/t/humann-fails-due-to-metaphlan-database-mismatch/16501/2):
>
> Hello @sri_microbiome
> 
> To incorporate HUMAnN with MetaPhlan, please try following the protocol outlined in this tutorial and associated workflows!
> 
> - 🎓 [Hands-on: Metatranscriptomics analysis using microbiome RNA-seq data / Metatranscriptomics analysis using microbiome RNA-seq data / Microbiome](https://training.galaxyproject.org/training-material/topics/microbiome/tutorials/metatranscriptomics/tutorial.html)
> 
> The reads are prepared, then sent through both tool pipelines independently, then the results from each are merged together after. This is what allows the reference data sources to work together.
> 
> Hope this helps! 🙂

Please give this a try! 🚀

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