Hi! Im working on a filter to remove from VCF file reads with QUAL<40, I run VCF with Hg19 genome on a VCF generated by FreeBayes for variant calling, after aligning with bowtie2, I leave all the parameters as default an in the first filter option I write -f “QUAL>40” (I require a treshold of 40) but I get the following error:
An error occurred with this dataset:
cannot subtract objects of dissimilar types 4 4
What am I doing wrong?