# VGP- unable to select or add hind3 restriction enzyme to Hi-C scaffolding workflow

**URL:** <https://help.galaxyproject.org/t/vgp-unable-to-select-or-add-hind3-restriction-enzyme-to-hi-c-scaffolding-workflow/13406>\
**Category:** usegalaxy.eu support\
**Tags:** workflow, workflow-options, vgp, hic\
**Created:** [September 10, 2024, 3:47pm UTC](https://help.galaxyproject.org/t/vgp-unable-to-select-or-add-hind3-restriction-enzyme-to-hi-c-scaffolding-workflow/13406 "2024-09-10T15:47:43Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![HT87](https://avatars.discourse-cdn.com/v4/letter/h/6de8d8/32.png) [@HT87](https://help.galaxyproject.org/u/HT87)\
**Post date:** [September 10, 2024, 3:47pm UTC](https://help.galaxyproject.org/t/vgp-unable-to-select-or-add-hind3-restriction-enzyme-to-hi-c-scaffolding-workflow/13406/1 "2024-09-10T15:47:43Z")

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Hello,

I noticed that VGP Hi-C scaffolding workflow does provide few selections for restriction enzyme input and does not allow a custom input. I used HindIII in my Hi-C library which have recognition site on AAGCTT.  
However, i can not input that to VGP Hi-C scaffolding workflow.

Any advice or help to resolve that would be appreciated.

Thank you

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<div class="post-metadata">

**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [September 10, 2024, 7:29pm UTC](https://help.galaxyproject.org/t/vgp-unable-to-select-or-add-hind3-restriction-enzyme-to-hi-c-scaffolding-workflow/13406/2 "2024-09-10T19:29:39Z")

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Welcome, @HT87

You can add in a custom input for this option – let me show you how to do this with that exact step!

I’m going to list all the steps to hopefully make this clear, and please ask followup questions. 🙂

1. **Import the workflow into your account**

2. **Open the workflow in the Editor**

3. **Locate the target tool**

In the screenshot, I followed the input for the “Preconfigured enzymes”, and found that it connected to the YAHS tool. Then I clicked on the YAHS tool in the canvas, and that action brought up the tool’s parameter settings in the right panel.

 ![Screen Shot 2024-09-10 at 11.36.54 AM](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/f/f31433691f0e14a9962e8e7b5fa7472c462aa45e.png)

1. **Review the available options for the parameter**

Screenshot of the available parameter settings.

![Screen Shot 2024-09-10 at 11.37.15 AM](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/7/71ae7e270e799809c377005ab4f048b772aa137e.png)

1. **Change the parameter setting**

Screenshot after the new parameter setting is selected: Enter a specific sequence. Notice that this is a free-text entry field. You could modify the workflow directly here and save, but that doesn’t make the workflow very reusable!

 ![Screen Shot 2024-09-10 at 11.37.35 AM](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/b/b78d50b72b14d0bbfe5831fd05aaca81b928342e.png)

1. **Toggle the option to add in a new input to the workflow**

Screenshot of where to set the toggle to instead add in a new place for a distinct input to this tool, and the workflow itself! This will be a new text file that will be selected when the workflow is run.

![Screen Shot 2024-09-10 at 11.38.31 AM](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/3/30051ca531bfda9c29dde9ae8bf748211e9dd14b.png)

Screenshot of the YAHS tool on the canvas, after that option is toggled.

 ![Screen Shot 2024-09-10 at 11.38.59 AM](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/c/c0f80825bb097d2a9ddbd1ca666a97105dfc467a.png)

1. **Adjust the workflow _inputs_ to match your new parameter settings!**

2. **Test your updated workflow!**

3. **Bonuses**

- More options for using custom workflow parameters are here: Galaxy Tutorial → [Hands-on: Using Workflow Parameters / Using Workflow Parameters / Using Galaxy and Managing your Data](https://training.galaxyproject.org/training-material/topics/galaxy-interface/tutorials/workflow-parameters/tutorial.html)

- You can also add in graphics to the workflow canvas using the palette functions. Examples of what that can look like are in these screenshots, and other places. This is entirely custom, and a great way to annotate details: where and what, _exactly_! Galaxy Tutorial → [Hands-on: Creating high resolution images of Galaxy Workflows / Creating high resolution images of Galaxy Workflows / Using Galaxy and Managing your Data](https://training.galaxyproject.org/training-material/topics/galaxy-interface/tutorials/workflow-posters/tutorial.html#hands-on-hiding-the-galaxy-ui-for-a-workflow).

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Please give that a try 🙂