# VGP - Workflow 1: K-mer Profiling - Need 2 PacBio Hifi Files But I Only Have 1

**URL:** <https://help.galaxyproject.org/t/vgp-workflow-1-k-mer-profiling-need-2-pacbio-hifi-files-but-i-only-have-1/15280>\
**Category:** Uncategorized\
**Tags:** workflow, assembly, vgp\
**Created:** [April 22, 2025, 11:45pm UTC](https://help.galaxyproject.org/t/vgp-workflow-1-k-mer-profiling-need-2-pacbio-hifi-files-but-i-only-have-1/15280 "2025-04-22T23:45:12Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![adswann](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/adswann/32/7098_2.png) [@adswann](https://help.galaxyproject.org/u/adswann)\
**Post date:** [April 22, 2025, 11:45pm UTC](https://help.galaxyproject.org/t/vgp-workflow-1-k-mer-profiling-need-2-pacbio-hifi-files-but-i-only-have-1/15280/1 "2025-04-22T23:45:12Z")

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Hello!

I’m an undergrad student, so please excuse me if there is a simple solution to this I’m not seeing.

I’m trying to run the [first workflow–K-mer profiling–](https://dockstore.org/workflows/github.com/iwc-workflows/kmer-profiling-hifi-VGP1/main:main?tab=info)in the [VGP-Galaxy Assembly Workflows Series](https://galaxyproject.org/projects/vgp/workflows/) by following [this tutorial](https://galaxyproject.org/projects/vgp/workflows/). The very first step of the K-mer profile analysis workflow says that it needs HiFi reads as a collection, but I only have one Hifi for my species. I can’t figure out how I am supposed to even start if I can’t get past the first step.

Any help would be much appreciated!!

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [April 22, 2025, 11:53pm UTC](https://help.galaxyproject.org/t/vgp-workflow-1-k-mer-profiling-need-2-pacbio-hifi-files-but-i-only-have-1/15280/2 "2025-04-22T23:53:40Z")

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Hi @adswann

To make sure I understand: a workflow that you want to use expects a collection input but you only have one sample. Yes?

If so, you can create a collection with just one sample. Follow the same building method.

Hope this helps and let us know if this works out or if you need more help. 🙂

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**Author:** ![adswann](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/adswann/32/7098_2.png) [@adswann](https://help.galaxyproject.org/u/adswann)\
**Post date:** [April 23, 2025, 12:11am UTC](https://help.galaxyproject.org/t/vgp-workflow-1-k-mer-profiling-need-2-pacbio-hifi-files-but-i-only-have-1/15280/3 "2025-04-23T00:11:30Z")

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Hi!

Thank you, that did work! I don’t know why I didn’t think to try making a collection with just the one file… 😅 I appreciate the help!!

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [April 23, 2025, 4:44pm UTC](https://help.galaxyproject.org/t/vgp-workflow-1-k-mer-profiling-need-2-pacbio-hifi-files-but-i-only-have-1/15280/4 "2025-04-23T16:44:10Z")

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No worries! Thinking about the big details and getting stuck on the small ones is sort of an occupational hazard in bioinformatics! Any questions are welcome here, no judgement. 🙂

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [April 18, 2026, 1:32am UTC](https://help.galaxyproject.org/t/vgp-workflow-1-k-mer-profiling-need-2-pacbio-hifi-files-but-i-only-have-1/15280/5 "2026-04-18T01:32:00Z")

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