Volcano plot error: Exit code 127

I attempted to generate a volcano plot using a tabular file produced by “Annotate DESeq2/DEXSeq output tables,” and the tool gave me this error message. Earlier today, I had generated volcano plots from unannotated DESeq2 result files just fine, so I find this really strange. Any help would be appreciated.

Justin

Hello @Justin_Liu

This looks like the cluster node failed to build the job environment. Please try a rerun to see if that is enough and let us know if you need more help! You could share back the technical details for the job (URL to this job details view with the logs would be enough). :slight_smile:

Hi @jennaj

I ran a rerun, and the same error message appeared. I’ve attached the URL to the history, please let me what I can do to fix it. Thank you.

History

Hello @Justin_Liu

Thanks for giving this a test!

It turns out that we were able to correct the dependencies for this tool, but then another version was just released, and that new one has the problem instead! Whew. We’ll sort this out.

But you don’t need to wait! Instead, please try to navigate to the prior version of the tool and use that instead. How to do this exactly is in the open issue ticket here. → Bug: Dependency issue with volcanoplot 4.0.1+galaxy0 · Issue #1123 · galaxyproject/usegalaxy-tools · GitHub

I just updated the ticket and completed my testing a few hours ago but please let us know if you can also get this to actually work.

Thanks for all of the follow up! :slight_smile:

Hello @jennaj

The tool works just fine now, thank you so much!

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