# What are the RNA-seq data processing steps in Galaxy according to de novo approach?

**URL:** <https://help.galaxyproject.org/t/what-are-the-rna-seq-data-processing-steps-in-galaxy-according-to-de-novo-approach/3943>\
**Category:** Uncategorized\
**Tags:** gtn-tutorial, salmon\
**Created:** [June 18, 2020, 4:45pm UTC](https://help.galaxyproject.org/t/what-are-the-rna-seq-data-processing-steps-in-galaxy-according-to-de-novo-approach/3943 "2020-06-18T16:45:39Z")\
**Posts on this page:** 1\
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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [June 19, 2020, 7:22pm UTC](https://help.galaxyproject.org/t/what-are-the-rna-seq-data-processing-steps-in-galaxy-according-to-de-novo-approach/3943/2 "2020-06-19T19:22:16Z")

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Hi @f_kurt

Please see this prior Q&A, it covers most of what you are asking about. _It is worth reviewing first_: [What to do with trinity output files](https://help.galaxyproject.org/t/what-to-do-with-trinity-output-files/2497)

For tools that appear to be missing, it could be that the Galaxy server you are working at does not have them installed. Or, the tool panel search is not finding them for some reason (search term typo?). You can also directly check.

- `Trimmomatic` is often under the tool group “FASTQ Quality Control”. And yes, performing QA is probably a good idea. `FastQC` is just a quality report – no changes are made to the data. It is important that both ends of paired-end data are input to assembly, and `Trimmomatic` will output pairs that are still paired post-QA.
- `Assemblystats` is available in the [ToolShed](https://toolshed.g2.bx.psu.edu/view/nml/assemblystats/7556309ffbaf) but is not hosted at all public Galaxy servers.
- `Fasta Statistics` is hosted and is usually under the tool group “FASTA/FASTQ”. Or, you may be more interested in `Compute contig Ex90N50 statistic and Ex90 transcript count from a Trinity assembly` in the tool group “RNA Analysis”.

The “Transcriptomics” Topic of the GTN Tutorials covers RNA-seq analysis (but not for all of this exact analysis – is still a work in progress – and explained in the linked Q&A above):

> [@Troubleshooting resources for errors or unexpected results](https://help.galaxyproject.org/t/troubleshooting-resources-for-errors-or-unexpected-results/42/1):
>
> [Galaxy Training Network Tutorials](https://galaxyproject.github.io/training-material/): Some [GTN](https://galaxyproject.org/teach/gtn/) tutorials are appropriate for [Galaxy Main](https://usegalaxy.org) and some are not. Where you can run each is noted per tutorial – click on the _Galaxy instances_ gear icon ![galaxyserversGTN](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/1X/1287f50cfcdaa1aad750fe323f96995260ac4947.png) to review the [Public Galaxy](https://galaxyproject.org/use/) server choices. If a tutorial is supported by a pre-configured [Galaxy Docker](https://hub.docker.com/r/bgruening/galaxy-stable/) training image, instructions for how to get it will be listed below the tutorial listings, per category.

Thanks!

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_[View the full topic](https://help.galaxyproject.org/t/what-are-the-rna-seq-data-processing-steps-in-galaxy-according-to-de-novo-approach/3943)._