I’m kind of new at this. I’ve only worked with paired Illumina data before and I now have Ab1 files that I need to work with. I’ve tried to convert to fasta and fastq files and work with those, but eventually I start getting error messages because it is never quite the format that the programs want. A lot of them want fastqsanger.gz files, and the files I have are from sanger sequencing. I’m not sure how to go further. I would like to be able to align the individual sequences in each pair to each other. Apart from that, I want to trim, assemble and blast, and then eventually make a phylogenetic tree. What is the best way to do this from Ab1 files?