# zero scores for rrnS and rrnL genes in MITOS2

**URL:** <https://help.galaxyproject.org/t/zero-scores-for-rrns-and-rrnl-genes-in-mitos2/12944>\
**Category:** usegalaxy.eu support\
**Tags:** genome-annotation\
**Created:** [July 11, 2024, 2:49pm UTC](https://help.galaxyproject.org/t/zero-scores-for-rrns-and-rrnl-genes-in-mitos2/12944 "2024-07-11T14:49:02Z")\
**Posts on this page:** 1\
**Showing post:** 6

<div class="post-metadata">

**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [July 15, 2024, 7:04pm UTC](https://help.galaxyproject.org/t/zero-scores-for-rrns-and-rrnl-genes-in-mitos2/12944/6 "2024-07-15T19:04:33Z")

</div>

Hi @BShelb

I’m not clear on which score is zero … but I did start up a test run to make sure the tool was working as expected. Maybe this example history will help, too?

See → [https://usegalaxy.eu/u/jenj/h/test-mitos](https://usegalaxy.eu/u/jenj/h/test-mitos)

The primary [BED](https://genome.ucsc.edu/FAQ/FAQformat.html#format1) output has content (a statistic in the “score” 5th column).

- _Tools:_ both **MITOS** and **MITOS2**

- _Inputs/parameters:_ I used all defaults with a super small test file for the first run’s output, then I ran it again and chose to output all of the possible results.

- _Results:_ The “score” field in the BED is the **eValue** from BLASTN. That value works a bit differently than a BLASTN score value. For eValue, “smaller is better” (more significant\*\*). If the value is too small to write out, then it gets rounded off by BLAST itself. For this case, it flows out to the output from MITOS/2.

All of it seems fine to me but let us know if you have questions. 🙂

---

_[View the full topic](https://help.galaxyproject.org/t/zero-scores-for-rrns-and-rrnl-genes-in-mitos2/12944)._