# \#busco **URL:** https://help.galaxyproject.org/tag/busco/495.md [Latest](https://help.galaxyproject.org/latest.md) · [Categories](https://help.galaxyproject.org/categories.md) · [Tags](https://help.galaxyproject.org/tags.md) --- ## [Funannotate BUSCO models - mucorales missing](https://help.galaxyproject.org/t/funannotate-busco-models-mucorales-missing/18386)
**Author:** [@Sydney\_Harned](https://help.galaxyproject.org/u/Sydney_Harned)\ **Replies:** 1\ **Last updated:** [September 14, 2026, 5:54pm UTC](https://help.galaxyproject.org/t/funannotate-busco-models-mucorales-missing/18386 "2026-09-14T17:54:43Z")
Hi Galaxy team, I’m running the “Funannotate predict annotation” tool and the “BUSCO models to align” dropdown only shows Dikarya as an option. Could you please add mucorales\_odb10 to the available lineages for this too… --- ## [Busco string\_type error](https://help.galaxyproject.org/t/busco-string-type-error/18060)
**Author:** [@Joseph\_Kogan](https://help.galaxyproject.org/u/Joseph_Kogan)\ **Replies:** 1\ **Last updated:** [August 10, 2026, 7:17pm UTC](https://help.galaxyproject.org/t/busco-string-type-error/18060 "2026-08-10T19:17:43Z")
I am trying to run BUSCO on a protein fasta file generated from a gff3 annotation, which has worked without a hitch in the past. This time, no matter what I do and regardless of the fact that I am using the exact same to… --- ## [Linking BUSCO genes to GO/functional terms for genome](https://help.galaxyproject.org/t/linking-busco-genes-to-go-functional-terms-for-genome/8037)
**Author:** [@ssell](https://help.galaxyproject.org/u/ssell)\ **Replies:** 1\ **Last updated:** [April 8, 2026, 1:34am UTC](https://help.galaxyproject.org/t/linking-busco-genes-to-go-functional-terms-for-genome/8037 "2026-04-08T01:34:49Z")
Hello, I have run BUSCO on galaxy and have the full table output in tabular form with the BUSCO genes identified. This includes the gene name, the coordinates, and a link to the OrthoDB page for that gene. Is there a w… --- ## [BUSCO + miniprot fails with No such file or directory](https://help.galaxyproject.org/t/busco-miniprot-fails-with-no-such-file-or-directory/17495)
**Author:** [@ccoulombe](https://help.galaxyproject.org/u/ccoulombe)\ **Replies:** 3\ **Last updated:** [February 24, 2026, 8:09pm UTC](https://help.galaxyproject.org/t/busco-miniprot-fails-with-no-such-file-or-directory/17495 "2026-02-24T20:09:56Z")
Hello, Tested on UseGalaxy.org and UseGalaxy.ca. Running BUSCO (5.8.0+galaxy2) fails with FileNotFoundError: \[Errno 2\] No such file or directory: '/cvmfs/data.galaxyproject.org/byhand/busco/v5/lineages/archaea\_odb10/re… --- ## [BUSCO error: refseq db not found](https://help.galaxyproject.org/t/busco-error-refseq-db-not-found/16328)
**Author:** [@H1889](https://help.galaxyproject.org/u/H1889)\ **Replies:** 8\ **Last updated:** [December 10, 2025, 10:49pm UTC](https://help.galaxyproject.org/t/busco-error-refseq-db-not-found/16328 "2025-12-10T22:49:52Z")
I ran busco to evaluate the draft of a bacterial genome and got this error: “FileNotFoundError: \[Errno 2\] No such file or directory: ‘/cvmfs/data.galaxyproject.org/byhand/busco/v5/lineages/bacilli\_odb10/refseq\_db.faa.gz… --- ## [Busco problem with choosing lineage](https://help.galaxyproject.org/t/busco-problem-with-choosing-lineage/15290)
**Author:** [@bioc\_dae](https://help.galaxyproject.org/u/bioc_dae)\ **Replies:** 3\ **Last updated:** [April 28, 2025, 10:01am UTC](https://help.galaxyproject.org/t/busco-problem-with-choosing-lineage/15290 "2025-04-28T10:01:52Z")
Dear Galaxy Team, I wanted to use the busco tool with the lineage of ascomycetes but when I try to choose a lineage, it says “No options available.” Could you please have a look at that? Thank you so much --- ## [Busco tool keeps crashing](https://help.galaxyproject.org/t/busco-tool-keeps-crashing/15194)
**Author:** [@yamkela\_mgwatyu](https://help.galaxyproject.org/u/yamkela_mgwatyu)\ **Replies:** 9\ **Last updated:** [April 15, 2025, 11:33am UTC](https://help.galaxyproject.org/t/busco-tool-keeps-crashing/15194 "2025-04-15T11:33:38Z")
Hi there, I am trying to run BUSCO and it just keeps on crashing. Is there an issue with the tool? This started yesterday, I’ve been running BUSCO with no issues before then. Could you please look into this? Also, it d… --- ## [Busco issues while running](https://help.galaxyproject.org/t/busco-issues-while-running/14650)
**Author:** [@Ines\_G\_Luque](https://help.galaxyproject.org/u/Ines_G_Luque)\ **Replies:** 1\ **Last updated:** [February 4, 2025, 9:40pm UTC](https://help.galaxyproject.org/t/busco-issues-while-running/14650 "2025-02-04T21:40:05Z")
Hey there! I am trying to run busco in protein mode, as I recently assembled a genome and now I’m working on it. However, no matter how I change the parameters, I always end up with the error: |2025-02-04 11:24:30 ERROR… --- ## [BUSCO for bacterial genome assemblies](https://help.galaxyproject.org/t/busco-for-bacterial-genome-assemblies/14475)
**Author:** [@Touraj\_Rahimi](https://help.galaxyproject.org/u/Touraj_Rahimi)\ **Replies:** 1\ **Last updated:** [January 17, 2025, 5:40pm UTC](https://help.galaxyproject.org/t/busco-for-bacterial-genome-assemblies/14475 "2025-01-17T17:40:37Z")
Hi, I’m trying to run BUSCO for some bacterial genome assemblies, in this case prodigal use as default gene prediction, but in galaxy eu, choosing one of three Select a gene predictor options which uses mainly for eukary… --- ## [BUSCO Fatal error: Exit code 1 ()](https://help.galaxyproject.org/t/busco-fatal-error-exit-code-1/13544)
**Author:** [@mmontonerin](https://help.galaxyproject.org/u/mmontonerin)\ **Replies:** 4\ **Last updated:** [September 27, 2024, 1:22pm UTC](https://help.galaxyproject.org/t/busco-fatal-error-exit-code-1/13544 "2024-09-27T13:22:55Z")
I am trying to run BUSCO on different genome assemblies, but I keep getting the error: Fatal error: Exit code 1 () The log doesn’t give any descriptive information of the problem: | 2024-09-25 15:08:55 ERRO… --- ## [BUSCO unable to run in offline mode?](https://help.galaxyproject.org/t/busco-unable-to-run-in-offline-mode/12950)
**Author:** [@jasper.rees](https://help.galaxyproject.org/u/jasper.rees)\ **Replies:** 1\ **Last updated:** [July 12, 2024, 9:02pm UTC](https://help.galaxyproject.org/t/busco-unable-to-run-in-offline-mode/12950 "2024-07-12T21:02:50Z")
Hi, I have a couple of failed runs with Busco since the 24.2 upgrade? The error is: 2024-07-11 22:35:41 ERROR: Unable to run BUSCO in offline mode. Dataset /data/db/data\_managers/busco/eukaryota+2024-03-23-082359/…