# \#fastq-format-error

**URL:** https://help.galaxyproject.org/tag/fastq-format-error/240.md

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## [UCSC Reference Genome and GTF Fatal Error no valid exons in the GTF file](https://help.galaxyproject.org/t/ucsc-reference-genome-and-gtf-fatal-error-no-valid-exons-in-the-gtf-file/14367)

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**Author:** [@drcottoncandy](https://help.galaxyproject.org/u/drcottoncandy)\
**Replies:** 3\
**Last updated:** [January 22, 2025, 7:41pm UTC](https://help.galaxyproject.org/t/ucsc-reference-genome-and-gtf-fatal-error-no-valid-exons-in-the-gtf-file/14367 "2025-01-22T19:41:32Z")

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Good morning! I am performing RNAstar and feature counts on the species camponotus floridanus. I have followed the tutorial training for RNAseq analysis of d.mel provided by Galaxy, and I had no issues. In this tutorial…

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## [De-Multiplexing with Je-Demultiplex-Illu](https://help.galaxyproject.org/t/de-multiplexing-with-je-demultiplex-illu/13784)

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**Author:** [@jkagle](https://help.galaxyproject.org/u/jkagle)\
**Replies:** 1\
**Last updated:** [October 30, 2024, 5:20pm UTC](https://help.galaxyproject.org/t/de-multiplexing-with-je-demultiplex-illu/13784 "2024-10-30T17:20:18Z")

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I just tried to use Je-Demultiplex-Illu to demultiplex my Illumina reads. I thought I entered everything correctly, but I got the following error. Any suggestions how to fix this? Picked up \_JAVA\_OPTIONS: -Xmx4G -Xms256…