# \#picrust2\_metagenome\_pipeline

**URL:** https://help.galaxyproject.org/tag/picrust2-metagenome-pipeline/663.md

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## [PICRUSt2 Metagenome prediction](https://help.galaxyproject.org/t/picrust2-metagenome-prediction/17431)

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**Author:** [@Peter\_Janampa](https://help.galaxyproject.org/u/Peter_Janampa)\
**Replies:** 3\
**Last updated:** [February 12, 2026, 8:06pm UTC](https://help.galaxyproject.org/t/picrust2-metagenome-prediction/17431 "2026-02-12T20:06:49Z")

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Hello, is there an example of an input based on the shared format? Specifically, should the shared file also contain FASTA sequences?

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## [Newick tree with study sequences placed amongst reference sequences](https://help.galaxyproject.org/t/newick-tree-with-study-sequences-placed-amongst-reference-sequences/15596)

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**Author:** [@Jeyamalar\_Jeyanathan](https://help.galaxyproject.org/u/Jeyamalar_Jeyanathan)\
**Replies:** 1\
**Last updated:** [July 25, 2025, 9:10pm UTC](https://help.galaxyproject.org/t/newick-tree-with-study-sequences-placed-amongst-reference-sequences/15596 "2025-07-25T21:10:25Z")

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How to build “Newick tree with study sequences placed amongst reference sequences” tree in galaxy for Picrusr2?