# \#ruvseq

**URL:** https://help.galaxyproject.org/tag/ruvseq/530.md

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## [RUVSeq:Fatal error: An undefined error occurred, please check your input carefully and contact your administrator](https://help.galaxyproject.org/t/ruvseq-fatal-error-an-undefined-error-occurred-please-check-your-input-carefully-and-contact-your-administrator/15254)

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**Author:** [@giovanna\_morello](https://help.galaxyproject.org/u/giovanna_morello)\
**Replies:** 1\
**Last updated:** [April 18, 2025, 5:37pm UTC](https://help.galaxyproject.org/t/ruvseq-fatal-error-an-undefined-error-occurred-please-check-your-input-carefully-and-contact-your-administrator/15254 "2025-04-18T17:37:10Z")

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I’ve been trying to use RUVSeq ( Remove Unwanted Variation from RNA-seq data) through inputting Salmon files. I have selected the corresponding files for each factor and made sure to select the Salmon option in the setti…

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## [Normalizing RNA-seq with RUVIII - PRPS](https://help.galaxyproject.org/t/normalizing-rna-seq-with-ruviii-prps/13681)

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**Author:** [@olddog1](https://help.galaxyproject.org/u/olddog1)\
**Replies:** 2\
**Last updated:** [October 16, 2024, 9:36pm UTC](https://help.galaxyproject.org/t/normalizing-rna-seq-with-ruviii-prps/13681 "2024-10-16T21:36:57Z")

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Hello - does anyone have experience with RNA-seq normalization using RUVIII PRPS? \* PMCID: \[PMC9849124\] Removing unwanted variation from large-scale RNA sequencing data with PRPS - PMC). Have an RNA-seq dataset that didn…