Hi, would like to use Galaxy to build an eDNA workflow pipeline to process Metabarcoding data from Illumina. Is this possible or are there other open source tools available to do this ? Basic steps needed : Read demultiplexed FASTQ files Merge paired reads Filter and trim Cluster Map to BINs i…

Hi @Adam_Hillier We have some community tutorials here that can get you started. Many will have a workflow template that you could build on, or these can at least serve as examples. Ecology / Tutorial List If you are new to Galaxy, this my favorite simple tutorial to start with. Hands-on: Gala…

⅕Thanks Jennifer and Galaxy support team, I am quite new to DNA. Completely self taught. But have made a lot of progress in 18 months. I think open access science is fantastic. I currently use mBRAVE. I don’t know if Galaxy is right tool for what I do but happy to find out and share feedback. Fee…

Hi @Adam_Hillier Great, glad you are exploring! For anyone else reading, this is one of the workflows in the Ecology group posted above. Maybe useful for comparisons between methods. :scientist: Hands-on: Metabarcoding/eDNA through Obitools / Metabarcoding/eDNA through Obitools / Ecology Thanks! …

Hi @Adam_Hillier , Thank you for exploring Galaxy solutions for eDNA analysis! And big thank you @jennaj for precise informations related notably to the work we are doing thanks to the Galaxy Ecology initiative! I think of interest to add links to these Galaxy training materials dedicated to metaba…