Hi all, i am having issues with the Mageck count feature in Galaxy. I uploaded my CRISPR Screen NGS paired end data in fastsanger format, performed Trim Galore! and Mageck count. The following error occurs: Details Execution resulted in the following messages: Fatal error: Exit code 255 () To…

Hello, This is probably the most informative part of the error message. It looks like a content or parameter problem that you have now solved. INFO @ Tue, 14 Feb 2023 11:23:18: Total tested reads: 100001, mapped: 0(0.0) ERROR @ Tue, 14 Feb 2023 11:23:18: Cannot automatically determine the --trim…

Hello Jennaj! Thank you very much for your reply! I do not fully understand how to solve the problem yet. Could you explain which parameters might have to be changed in order to solve the issue? The error still occurs: INFO @ Wed, 15 Feb 2023 12:06:29: Total tested reads: 100001, mapped: 6(5.99…

[image] boegerm: I do not fully understand how to solve the problem yet. Could you explain which parameters might have to be changed in order to solve the issue? There were successful runs in the shared history, which means that the technical issues around trimming were resolved and now the i…

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Exit code 255 in Mageck Count

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