Dear Galaxy users, I am trying to analyse some 16S rRNA data following the Galaxy tutorial. When i run the unique.seqs call i get the following message: An error occured while running the tool toolshed.g2.bx.psu.edu/repos/iuc/mothur_unique_seqs/mothur_unique_seqs/1.39.5.0. Tool execution generate…

Ok, that does help to confirm that memory is the root problem. Please see the Galaxy administrator help for how to increase memory allocation for tools. This tuning is usually most appropriate for those that are running jobs on a cluster, which might not be your case. By default, when running Gala…

It looks like the tool is running out of memory during the job processing. This could be because of mixed-up inputs or using inputs that are too large to execute on your Galaxy server. Has the tool worked before or is the first time using it? You might want to double check that the Mothur tool sui…

Dear jennaj , Many thanks for your reply. I have run before this command without any problems. Do you mean that my RAM is running out? Shall i try to break down the dataset to smaller datasets and then merge the data? Is that possible? Thanks a lot for your kind help Best N [image]

There could be a format/content problem with the input or the job is really running out of memory during execution. Breaking the dataset up into smaller chunks would not work well with this tool. What happens if you run the same job at a public Galaxy server? You can try at Galaxy Main https://use…

Many thanks for your reply again. I might not be able to run the same job online as my dataset is large and i might exceed the available space. I am running again the same calls with a smaller dataset. Is there any way to increase the memory? [UPDATE] I finished running the same calls with a small…

Dear jennaj , I tried to use Galaxy.org and i got again the same problem. Are there any other options? Is there a way to segment the dataset and after the alignment pool the samples? This is a University Laboratory project on 16S rRNA. Best N

Galaxy Community Help

Unique.seqs fatal error 137 -- Tool/memory failure on a local Galaxy

devops-administration, galaxy-local, mothur

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