Fairly new to all this DNA analysis (not my background), I’ve gone through the 16S Microbial Analysis with mothur (extended) tutorial and had no issues. I’ve now started work on my own data (analysing 16S RNA region from sediments) and followed the tutorial steps with my own data.
Now reaching the Align.seqs step I have used the larger Silva reference files downloaded from: Silva reference files
(release version 138.2, uncompressed file name silva.nr_v138_2.align)
Running the step returns with the Error 153 text:
/data/jwd05e/main/074/487/74487835/tool_script.sh: line 23: 18 Done echo ‘align.seqs( fasta=fasta.dat, reference=alignment.reference.dat, align=needleman, ksize=8, processors=’${GALAXY_SLOTS:-8}’ )’
19 | sed ‘s/ //g’
20 File size limit exceeded(core dumped) | ./mothur
21 | tee mothur.out.log
Understandably a larger file. A colleague had gone through this last year and used the same reference align file (theirs worked) so looking for help here!
Any suggestions?
Thanks you!