Using FastANI for determining average nucleotide identities (ANI) between potential species is a bit problematic, because it simply returns an empty result in cases where similarities are very low. It would be more helpful if it reported i.e. “no sufficient similarities found”, but it is what it is…
For this reason it would be nice to have an alternative tool for ANI-comparisons between potential species (e.g. PyANI), but i can’t seem to find them amon the available usegalaxy.eu tools.
could anyone name some alternatives that ARE available here? Or could alternatives like PyANI still be implemented? Also maybe this behaviour should be documented on the FastANI tool-page?