This is an optional input. You can try either way – I haven’t seen any reported problems about it and it is used all the time by others. Or leave it blank and keep it simple. This is your own server so you can set it up any way you want.
Hey,
Just to finish this thread, the duplicate file was causing the issue with BamLeftAlign. Re-installing galaxy and being more careful with creating DBKey and indexes, and confirming there was no duplicates in Data Tables worked, and I was able to run BamLeftAlign successfully
Thanks again for your help!
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Super, glad that worked out and thanks for posting back!