Cuffmerge error: duplicate/invalid 'transcript' feature ID=Ma03_t01040.3

Hi @tikshana_yadav

Some gff3 datasets are released by data providers that contained duplicated “ID” identifiers. These cause problems with almost all tools. Using gtf annotation is a better choice.

Please note that the tools you are using are deprecated. Details, reasons, and alternatives are covered in this prior Q&A:

Thanks!